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Characterization of Polymer–Enzyme Composite Biosensors for Brain Monitoring In Vivo

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Microelectrode Biosensors

Part of the book series: Neuromethods ((NM,volume 80))

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Abstract

The application of biosensors for near real-time monitoring of key energy and signaling molecules in the intact brain poses major challenges at a number of levels. In this context, amperometric biosensor functionality is impacted by a variety of physicochemical and biological phenomena, including enzyme substrate and co-substrate sensitivity, interference from endogenous electroactive species, fabrication and response consequences of miniaturization for tissue implantation, and biocompatibility issues. Polymer–enzyme composite (PEC) implantable microbiosensors, incorporating a poly-o-phenylenediamine (PoPD) ultrathin permselective barrier, possess a variety of characteristics which make them suitable for long-term in-vivo electrochemical (LIVE) monitoring. This chapter reviews the use of PoPD in this context, and describes a battery of PEC sensitivity and selectivity parameters which allow development of the basic design in a systematic way in order to understand and improve their performance, and to diversify the analyte range of these novel probes of brain function.

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Acknowledgements

This work was supported by funding from Science Foundation Ireland (SFI), the Irish Research Council for Science, Engineering and Technology (IRCSET), and by University College Dublin (UCD).

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  1. UCD School of Chemistry and Chemical Biology, University College Dublin, Belfield, Dublin 4, Ireland

    Robert D. O’Neill

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  1. Faculté de médecine, CRNL, Université Claude Bernard Lyon I, Avenue Rockefeller 8, Lyon, 69373, France

    Stéphane Marinesco

  2. School of Life Sciences, University of Warwick, Gibbet Hill Road, Coventry, CV4 7AL, United Kingdom

    Nicholas Dale

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O’Neill, R.D. (2013). Characterization of Polymer–Enzyme Composite Biosensors for Brain Monitoring In Vivo. In: Marinesco, S., Dale, N. (eds) Microelectrode Biosensors. Neuromethods, vol 80. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-370-1_6

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  • DOI: https://doi.org/10.1007/978-1-62703-370-1_6

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