Abstract
Cellular localization of proteins is one of the most valuable sources of information regarding spatiotemporal biological events involved in human disease. This information is sometimes enhanced by carrying out protein isolation using a process known as subcellular fractionation. This involves the sequential extraction of proteins from specific compartments and/or organelles within the cell. Additionally, subcellular fractionation for biomarker discovery enables the in-depth analysis of biomolecules by reducing the complexity of the protein mixture. In this chapter, four custom fractionation approaches and one commercial kit are compared for their efficacy and compatibility with subsequent proteomic analysis.
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Acknowledgments
Ms. Kimberly McKinney and Dr. Jin-Gyun Lee are gratefully acknowledged for their technical assistance and comments. This work was supported by Carolinas HealthCare System, a start-up package and a research grant (CMC 08-026).
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Hwang, SI., Han, D.K. (2013). Subcellular Fractionation for Identification of Biomarkers: Serial Detergent Extraction by Subcellular Accessibility and Solubility. In: Zhou, M., Veenstra, T. (eds) Proteomics for Biomarker Discovery. Methods in Molecular Biology, vol 1002. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-360-2_3
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DOI: https://doi.org/10.1007/978-1-62703-360-2_3
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-359-6
Online ISBN: 978-1-62703-360-2
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