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Mutant p53 in Cell Adhesion and Motility

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p53 Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 962))

Abstract

Pro-oncogenic properties of mutant p53 were investigated with the aid of migration assays, adhesion assays, and soft agar growth assays using cells stably expressing gain-of-function p53 mutants. To determine cell migration, “wound-healing” (scratch) assays and haptotactic (chamber) assays were used. H1299 cells expressing mutant p53 were found to migrate more rapidly than cells transfected with empty vector alone. Results from both types of migration assay were broadly similar. Migratory ability differed for different p53 mutants, suggesting allele-specific effects. Cells expressing p53 mutants also showed enhanced adhesion to extracellular matrix compare to controls. Furthermore, stable transfection of mutant p53-H179L into NIH3T3 fibroblasts was sufficient to allow anchorage-independent growth in soft agar.

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Acknowledgments

Studies reported herein were supported in part by pilot project grants from the Massey Cancer Center to W.A.Y. and S.D., and by NCI R01 to S.D.

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Authors and Affiliations

  1. Philips Institute of Oral & Craniofacial Molecular Biology, School of Dentistry, Virginia Commonwealth University, Richmond, VA, USA

    W. Andrew Yeudall

  2. Nature Reviews, Macmillan Press, London, UK

    Katharine H. Wrighton

  3. Department of Biochemistry, Massey Cancer Center, Department of Microbiology and Immunology, Virginia Commonwealth University, Richmond, VA, USA

    Sumitra Deb

Authors
  1. W. Andrew Yeudall
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  2. Katharine H. Wrighton
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  3. Sumitra Deb
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Corresponding author

Correspondence to Sumitra Deb .

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Editors and Affiliations

  1. Massey Cancer Center, Department of Biochemistry, Virginia Commonwealth University, College Street 401, Richmond, 23298, Virginia, USA

    Sumitra Deb

  2. Massey Cancer Center, Department of Biochemistry, Virginia Commonwealth University, College Street 401, Richmond, 23298, Virginia, USA

    Swati Palit Deb

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© 2013 Springer Science+Business Media New York

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Yeudall, W.A., Wrighton, K.H., Deb, S. (2013). Mutant p53 in Cell Adhesion and Motility. In: Deb, S., Deb, S. (eds) p53 Protocols. Methods in Molecular Biology, vol 962. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-236-0_11

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  • DOI: https://doi.org/10.1007/978-1-62703-236-0_11

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-235-3

  • Online ISBN: 978-1-62703-236-0

  • eBook Packages: Springer Protocols

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