Abstract
Autoimmune bullous diseases are the best-characterized autoimmune skin diseases. Molecular diagnosis of these diseases has become possible due to the identification of their target autoantigens over the past three decades. In this review, we summarize methodology for categorizing autoimmune bullous diseases by means of combinations of direct and indirect immunofluorescence techniques using normal human skin sections, rat bladder sections and COS7 cells transfected with desmocollins 1–3 encoded vectors, enzyme-linked immunosorbent assays and immunoblotting with normal human epidermal extracts, dermal extracts, purified proteins from cell cultures and recombinant proteins.
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Acknowledgment
We greatly appreciate Ms. Ayumi Suzuki, Ms. Takako Ishikawa, and Ms. Sachiko Sakaguchi for technical assistance, and Ms. Akiko Tanaka, Ms. Yasuko Nakayama, Ms. Emiko Hara, Ms. Hanako Tomita, Ms. Mihoko Ikeda, Ms. Kyoko Akashi, and Ms. Nobuko Ishii for secretarial work.
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Tsuruta, D., Dainichi, T., Hamada, T., Ishii, N., Hashimoto, T. (2013). Molecular Diagnosis of Autoimmune Blistering Diseases. In: Has, C., Sitaru, C. (eds) Molecular Dermatology. Methods in Molecular Biology, vol 961. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-227-8_2
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