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In-Field Diagnostics Using Loop-Mediated Isothermal Amplification

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Phytoplasma

Part of the book series: Methods in Molecular Biology ((MIMB,volume 938))

Abstract

Loop-mediated isothermal amplification (LAMP) is a method for amplification and detection of target organisms which, unlike polymerase chain reaction, does not require thermal cycling. LAMP assays can be developed in the laboratory for subsequent deployment in the field, where the simplicity of isothermal amplification makes LAMP a suitable method for rapid detection of phytoplasmas with levels of sensitivity and specificity approaching those of more complex and time-consuming laboratory methods.

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Author information

Authors and Affiliations

  1. The Food and Environment Research Agency, Sand Hutton, York, UK

    Jenny Tomlinson

Authors
  1. Jenny Tomlinson
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Corresponding author

Correspondence to Jenny Tomlinson .

Editor information

Editors and Affiliations

  1. School of Biosciences, The University of Nottingham, Sutton Bonington Campus, Nottingham, LE12 5RD, United Kingdom

    Matt Dickinson

  2. The Food and Environment Research Agency, Sand Hutton, YO41 1LZ, United Kingdom

    Jennifer Hodgetts

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Tomlinson, J. (2013). In-Field Diagnostics Using Loop-Mediated Isothermal Amplification. In: Dickinson, M., Hodgetts, J. (eds) Phytoplasma. Methods in Molecular Biology, vol 938. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-089-2_25

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  • DOI: https://doi.org/10.1007/978-1-62703-089-2_25

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-088-5

  • Online ISBN: 978-1-62703-089-2

  • eBook Packages: Springer Protocols

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