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Establishment of a Sanguinarine-Producing Cell Suspension Culture of Argemone mexicana L (Papaveraceae): Induction of Alkaloid Accumulation

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Plant Cell Culture Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 877))

Abstract

A protocol for the induction of a cell suspension culture of Argemone mexicana is described. This suspension has been kept for over 3 years producing sanguinarine, a benzophenanthridine-type alkaloid. Sanguinarine levels can be increased by exposing these cultures to yeast or fungal elicitation.

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References

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Author information

Authors and Affiliations

  1. Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, Mérida, Yucatán, México

    Felipe A. Vázquez-Flota, Miriam Monforte-González, Cecilia Guízar-González, Jorge Rubio-Piña & Karen Trujillo-Villanueva

Authors
  1. Felipe A. Vázquez-Flota
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  2. Miriam Monforte-González
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  3. Cecilia Guízar-González
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  4. Jorge Rubio-Piña
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  5. Karen Trujillo-Villanueva
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Corresponding author

Correspondence to Felipe A. Vázquez-Flota .

Editor information

Editors and Affiliations

  1. , Unidad de Bioquímica y Biología Molecula, Centro de Investigación Científica de Yu, Apartado Postal 87, Cordemex, Mérida, Mexico

    Víctor M. Loyola-Vargas

  2. , Departamento de Ingeniería Genética de P, Ctr Investigación, Estudios Avanzados In, Apartado Postal 629, Irapuato, 36821, Mexico

    Neftalí Ochoa-Alejo

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Vázquez-Flota, F.A., Monforte-González, M., Guízar-González, C., Rubio-Piña, J., Trujillo-Villanueva, K. (2012). Establishment of a Sanguinarine-Producing Cell Suspension Culture of Argemone mexicana L (Papaveraceae): Induction of Alkaloid Accumulation. In: Loyola-Vargas, V., Ochoa-Alejo, N. (eds) Plant Cell Culture Protocols. Methods in Molecular Biology, vol 877. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-818-4_21

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  • DOI: https://doi.org/10.1007/978-1-61779-818-4_21

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-817-7

  • Online ISBN: 978-1-61779-818-4

  • eBook Packages: Springer Protocols

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