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Assessing Rhoptry Secretion in T. gondii

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Toxoplasma gondii

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2071))

Abstract

Rhoptries are key secretory organelles for Toxoplasma gondii invasion. Here, we describe how to assess the ability of T. gondii tachyzoites to secrete their rhoptry contents in vitro.

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Acknowledgments

Our research on rhoptry secretion is supported by the Laboratoire d’Excellence (LabEx) (ParaFrap ANR-11-LABX-0024) and by the Fondation pour la Recherche Médicale (Equipe FRM EQ. 20170336725) to M.L. M.B.L. is supported by NIH R01AI20846. We would like to thank Julien Marcetteau for the graphic design of the figure and Anita Koshy for helpful comments.

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Authors and Affiliations

  1. UMR 5235 CNRS, Université de Montpellier, Montpellier, France

    Catherine Suarez & Maryse Lebrun

  2. Department of Molecular Biology and Biochemistry, University of California, Irvine, Irvine, CA, USA

    Melissa B. Lodoen

  3. Institute for Immunology, University of California, Irvine, Irvine, CA, USA

    Melissa B. Lodoen

Authors
  1. Catherine Suarez
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  2. Melissa B. Lodoen
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  3. Maryse Lebrun
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Corresponding author

Correspondence to Maryse Lebrun .

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Editors and Affiliations

  1. Division of Infectious Disease and Immune Defense, The Walter and Eliza Hall Institute of Medical Research, Melbourne, VIC, Australia

    Christopher J. Tonkin

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Suarez, C., Lodoen, M.B., Lebrun, M. (2020). Assessing Rhoptry Secretion in T. gondii. In: Tonkin, C. (eds) Toxoplasma gondii. Methods in Molecular Biology, vol 2071. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9857-9_8

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  • DOI: https://doi.org/10.1007/978-1-4939-9857-9_8

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9856-2

  • Online ISBN: 978-1-4939-9857-9

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