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Toxoplasma gondii

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2071)

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Table of contents (23 protocols)

  1. Front Matter

    Pages i-xi
  2. Isolation and Genotyping of Toxoplasma gondii Strains

    • Chunlei Su, Jitender P. Dubey
    Pages 49-80
  3. PCR Screening of Toxoplasma gondii Single Clones Directly from 96-Well Plates Without DNA Purification

    • Federica Piro, Vern B. Carruthers, Manlio Di Cristina
    Pages 117-123
  4. Assessing Rhoptry Secretion in T. gondii

    • Catherine Suarez, Melissa B. Lodoen, Maryse Lebrun
    Pages 143-155
  5. High-Throughput Measurement of Microneme Secretion in Toxoplasma gondii

    • Kevin M. Brown, L. David Sibley, Sebastian Lourido
    Pages 157-169
  6. Plate-Based Quantification of Stimulated Toxoplasma Egress

    • Emily Shortt, Sebastian Lourido
    Pages 171-186
  7. Genetic Indicators for Calcium Signaling Studies in Toxoplasma gondii

    • Stephen A. Vella, Abigail Calixto, Beejan Asady, Zhu-Hong Li, Silvia N. J. Moreno
    Pages 187-207
  8. Phenotyping Toxoplasma Invasive Skills by Fast Live Cell Imaging

    • Georgios Pavlou, Isabelle Tardieux
    Pages 209-220
  9. Experimental Approaches for Examining Apicoplast Biology

    • Marco Biddau, Jana Ovciarikova, Lilach Sheiner
    Pages 221-243
  10. Measuring Solute Transport in Toxoplasma gondii Parasites

    • Esther Rajendran, Kiaran Kirk, Giel G. van Dooren
    Pages 245-268
  11. Toxoplasma gondii: Bradyzoite Differentiation In Vitro and In Vivo

    • Joshua Mayoral, Manlio Di Cristina, Vern B. Carruthers, Louis M. Weiss
    Pages 269-282
  12. Three-Dimensional Reconstruction of Toxoplasma–Neuron Interactions In Situ

    • Carla M. Cabral, Emily F. Merritt, Anita A. Koshy
    Pages 283-295
  13. Model Systems for Studying Mechanisms of Ocular Toxoplasmosis

    • Justine R. Smith, Liam M. Ashander, Yuefang Ma, Elise Rochet, João M. Furtado
    Pages 297-321
  14. Using BioID for the Identification of Interacting and Proximal Proteins in Subcellular Compartments in Toxoplasma gondii

    • Peter J. Bradley, Shima Rayatpisheh, James A. Wohlschlegel, Santhosh M. Nadipuram
    Pages 323-346
  15. Assays to Evaluate Toxoplasma–Macrophage Interactions

    • Debanjan Mukhopadhyay, Jeroen P. J. Saeij
    Pages 347-370

About this book

This volume covers a diverse collection of protocols currently being used by the Toxoplasma research community, and also looks at innovative methods that are pushing the boundaries of possibilities. Chapters in this book discuss topics such as isolation and genotyping of Toxoplasma gondii strains; assessing rhoptry secretion in T. gondii; plate-based quantification of stimulated Toxoplasma egress; methods to study ocular toxoplasmosis; and metabolic analysis of Toxoplasma gondii tachyzoites. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Toxoplasma gondii: Methods and Protocols is a valuable resource for students or groups starting off in the field, as well as laboratories interested in implementing the latest techniques described in the book.



Editors and Affiliations

  • Division of Infectious Disease and Immune Defense, The Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia

    Christopher J. Tonkin

Bibliographic Information

  • Book Title: Toxoplasma gondii

  • Book Subtitle: Methods and Protocols

  • Editors: Christopher J. Tonkin

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9857-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-4939-9856-2Published: 23 November 2019

  • Softcover ISBN: 978-1-4939-9859-3Published: 23 November 2020

  • eBook ISBN: 978-1-4939-9857-9Published: 22 November 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 470

  • Number of Illustrations: 14 b/w illustrations, 63 illustrations in colour

  • Topics: Immunology

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access