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Protein Self-Assembly

Methods and Protocols

  • Jennifer J. McManus

Part of the Methods in Molecular Biology book series (MIMB, volume 2039)

Table of contents

  1. Front Matter
    Pages i-x
  2. Measuring Protein-Protein Interactions and Protein Phase Diagrams

    1. Front Matter
      Pages 1-1
    2. Daniel Corbett, Jordan W. Bye, Robin A. Curtis
      Pages 3-21
    3. Mahlet A. Woldeyes, Cesar Calero-Rubio, Eric M. Furst, Christopher J. Roberts
      Pages 23-37
    4. Neer Asherie
      Pages 51-57
  3. Measuring Protein Self-Association, Aggregation, and Crystallization

    1. Front Matter
      Pages 59-59
    2. Matthew R. Jacobs, Mark Grace, Alice Blumlein, Jennifer J. McManus
      Pages 117-129
    3. Svenja Sladek, Kate McComiskey, Anne Marie Healy, Lidia Tajber
      Pages 131-139
    4. Zahra Rattray, Egor Zindy, Kara M. Buzza, Alain Pluen
      Pages 141-155
    5. Judith J. Mittag, Matthew R. Jacobs, Jennifer J. McManus
      Pages 157-171
    6. Pancham S. Kandiyal, Ji Yoon Kim, Daniel L. Fortunati, K. H. Mok
      Pages 173-183
  4. Computational Approaches to Measure Protein Self-Assembly

    1. Front Matter
      Pages 185-185
    2. Nicoletta Gnan, Francesco Sciortino, Emanuela Zaccarelli
      Pages 187-208
    3. Irem Altan, Patrick Charbonneau
      Pages 209-227
    4. Deniz Meneksedag-Erol, Sarah Rauscher
      Pages 243-262
  5. Back Matter
    Pages 263-266

About this book

Introduction

This volume explores experimental and computational approaches to measuring the most widely studied protein assemblies, including condensed liquid phases, aggregates, and crystals. The chapters in this book are organized into three parts: Part One looks at the techniques used to measure protein-protein interactions and equilibrium protein phases in dilute and concentrated protein solutions; Part Two describes methods to measure kinetics of aggregation and to characterize the assembled state; and Part Three details several different computational approaches that are currently used to help researchers understand protein self-assembly. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and cutting-edge, Protein Self-Assembly: Methods and Protocols is a valuable resource for researchers who are interested in learning more about this developing field. 

Keywords

Globular Proteins protein unfolding mesophases microscopy capillary electrophoresis

Editors and affiliations

  • Jennifer J. McManus
    • 1
  1. 1.Department of ChemistryMaynooth UniversityMaynooth, Co. KildareIreland

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-9678-0
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9677-3
  • Online ISBN 978-1-4939-9678-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site