Abstract
In recent years, nanoparticle tracking analysis (NTA) has emerged as an alternative tool for particle size characterization. Especially when examining polydisperse systems, individual particle to particle tracking allows for higher peak resolution than dynamic light scattering techniques. However, NTA requires an experienced user with a good insight into how the different settings can affect the determination of particle size and size distributions. This chapter provides a guideline for protein aggregation studies using the example of temperature-induced aggregation of IgG at low concentration.
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Acknowledgments
This chapter has emanated from research conducted with the financial support of Enterprise Ireland under Grant Number [IP/2015 0358] and the Synthesis and Solid State Pharmaceutical Centre, funded by Science Foundation Ireland (SFI) under Grant Number [12/RC/2275], cofunded under the European Regional Development Fund.
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Sladek, S., McComiskey, K., Healy, A.M., Tajber, L. (2019). Nanoparticle Tracking Analysis to Examine the Temperature-Induced Aggregation of Proteins. In: McManus, J. (eds) Protein Self-Assembly. Methods in Molecular Biology, vol 2039. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9678-0_10
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DOI: https://doi.org/10.1007/978-1-4939-9678-0_10
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