Abstract
CRISPR/Cas9 technology allows facile modification of the genome in virtually any desired way through the use of easily designed plasmid constructs that express a gRNA targeting a genomic site-of-interest and Cas9. Hydrodynamic tail vein injection, on the other hand, is a simple method to deliver “naked” plasmid DNA to 5–40% of the hepatocytes of the liver of adult mice. Here, we describe how these two techniques can be combined to create a workflow for fast, easy, and cost-efficient in vivo genome editing of the adult mouse liver. Using this method, large cohorts of mice with genetically modified livers can be established within 3 weeks to generate models for gene function in normal physiology and diseases of the liver.
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Acknowledgments
This work was supported by the Danish Cancer Society (R146-A9563-B3551 to F.N. and R124-A7632-15-S2 to M.F.), the Danish Council for Independent Research (DFF-Mobilex 4092-00235 to F.N.), and the Novo Nordisk Foundation (NNF17OC0028380 to M.F.)
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Niola, F., Dagnæs-Hansen, F., Frödin, M. (2019). In Vivo Editing of the Adult Mouse Liver Using CRISPR/Cas9 and Hydrodynamic Tail Vein Injection. In: Luo, Y. (eds) CRISPR Gene Editing. Methods in Molecular Biology, vol 1961. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9170-9_20
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DOI: https://doi.org/10.1007/978-1-4939-9170-9_20
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