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CRISPR Gene Editing

Methods and Protocols

  • Yonglun Luo

Part of the Methods in Molecular Biology book series (MIMB, volume 1961)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Methods for CRISPR-gRNA Design and Quantification of Activity

    1. Front Matter
      Pages 1-1
    2. Maria Pallarès Masmitjà, Nastassia Knödlseder, Marc Güell
      Pages 3-11
    3. Lin Lin, Yonglun Luo
      Pages 13-28
    4. Eva Karina Brinkman, Bas van Steensel
      Pages 29-44 Open Access
    5. Saskia König, Zhang Yang, Hans Heugh Wandall, Claudio Mussolino, Eric Paul Bennett
      Pages 45-66
  3. Methods for CRISPR Delivery

    1. Front Matter
      Pages 79-79
    2. Suleixin Yang, Qinjie Wu, Yuquan Wei, Changyang Gong
      Pages 81-91
    3. Laura Barrett Ryø, Emil Aagaard Thomsen, Jacob Giehm Mikkelsen
      Pages 93-109
  4. CRISPR Gene Editing in Human iPSCs

    1. Front Matter
      Pages 135-135
    2. Saniye Yumlu, Sanum Bashir, Jürgen Stumm, Ralf Kühn
      Pages 137-151
    3. Michaela Bruntraeger, Meg Byrne, Kathleen Long, Andrew R. Bassett
      Pages 153-183
    4. Kirsten E. Snijders, James D. Cooper, Ludovic Vallier, Alessandro Bertero
      Pages 185-209
  5. CRISPR Gene Editing in Other Cell Types

    1. Front Matter
      Pages 211-211
    2. Daria Sergeeva, Jose Manuel Camacho-Zaragoza, Jae Seong Lee, Helene Faustrup Kildegaard
      Pages 213-232
    3. Signe Neldeborg, Lin Lin, Magnus Stougaard, Yonglun Luo
      Pages 233-247
    4. Lisbeth Ahm Hansen, Ernst-Martin Füchtbauer
      Pages 249-254
    5. Xi Xiang, Conghui Li, Xi Chen, Hongwei Dou, Yong Li, Xiuqing Zhang et al.
      Pages 255-269
    6. Petra Vochozkova, Kilian Simmet, Eva-Maria Jemiller, Annegret Wünsch, Nikolai Klymiuk
      Pages 271-289
  6. CRISPR Gene Therapy and Screening

    1. Front Matter
      Pages 291-291
    2. Kristian Alsbjerg Skipper, Jacob Giehm Mikkelsen
      Pages 293-306
    3. Andreas Holmgaard, Sidsel Alsing, Anne Louise Askou, Thomas J. Corydon
      Pages 307-328
    4. Francesco Niola, Frederik Dagnæs-Hansen, Morten Frödin
      Pages 329-341
  7. Back Matter
    Pages 359-362

About this book

Introduction

This detailed volume guides readers through strategic planning and user-friendly guidelines in order to select the most suitable CRISPR-Cas system and target sites with high activity and specificity. Methods covering CRISPR gRNA design, CRISPR delivery, CRISPR activity quantification (indel quantification), and examples of applying CRISPR gene editing in human pluripotent stem cells, primary cells, gene therapy, and genetic screening are included. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and invaluable, CRISPR Gene Editing: Methods and Protocols will assist undergraduates, graduates, and researchers with detailed guidelines and methods for the vitally important CRISPR gene editing field. 

Chapter 3 is available open access under a CC BY 4.0 license via link.springer.com.

Keywords

CRISPR-Cas Genome editing Genetic modification gRNA design Regenerative medicine Genetic manipulation tools CRISPR delivery Gene therapy

Editors and affiliations

  • Yonglun Luo
    • 1
  1. 1.Department of BiomedicineAarhus UniversityAarhusDenmark

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-9170-9
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-9169-3
  • Online ISBN 978-1-4939-9170-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site