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Quantification of Viable but Non-Culturable Cells of Legionella pneumophila

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Legionella

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1921))

Abstract

Legionella pneumophila, among other bacteria, may enter a viable but non-culturable state as a means for survival in stressful conditions. Bacterial cells in the viable but non-culturable state cannot grow on standard medium; however, they continue to exhibit characteristics that are associated with live cells, such as respiration, transcription, and cell wall integrity. The present paper outlines a detailed protocol for the detection of viable but non-culturable L. pneumophila cells via Syto® 9 and propidium iodide staining coupled with flow cytometry.

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Acknowledgments

This work was supported by Discovery Grant 418289-2012 from the National Sciences and Engineering Research Council of Canada (NSERC) and a John R. Evans Leaders Fund—Funding for research infrastructure from the Canadian Foundation for Innovation to SPF. NM was the recipient of a PhD scholarship from Fond de Recherche du Québec—Nature et Technologie.

Author information

Authors and Affiliations

  1. New York University College of Dentistry, New York, NY, USA

    Rosalie S. Braun

  2. Faculty of Agricultural and Environmental Sciences, Department of Natural Resource Sciences, McGill University, Ste-Anne-de-Bellevue, QC, Canada

    Rosalie S. Braun, Nilmini Mendis, Laam Li & Sebastien P. Faucher

  3. Department of Chemistry, City University of Hong Kong, Kowloon, Hong Kong

    Laam Li

Authors
  1. Rosalie S. Braun
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  2. Nilmini Mendis
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  3. Laam Li
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  4. Sebastien P. Faucher
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Corresponding author

Correspondence to Sebastien P. Faucher .

Editor information

Editors and Affiliations

  1. Institut Pasteur, Biologie des Bactéries Intracellulaires, Paris, France

    Carmen Buchrieser

  2. Institute of Medical Microbiology, University of Zürich, Zürich, Switzerland

    Hubert Hilbi

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Braun, R.S., Mendis, N., Li, L., Faucher, S.P. (2019). Quantification of Viable but Non-Culturable Cells of Legionella pneumophila. In: Buchrieser, C., Hilbi, H. (eds) Legionella. Methods in Molecular Biology, vol 1921. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9048-1_3

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  • DOI: https://doi.org/10.1007/978-1-4939-9048-1_3

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9047-4

  • Online ISBN: 978-1-4939-9048-1

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