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Recombinant CHO Cell Pool Generation Using piggyBac Transposon System

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Recombinant Protein Expression in Mammalian Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1850))

Abstract

CHO cell pools with desirable characteristics of high titer and consistent product quality are useful for rapid production of recombinant proteins. Here we describe the generation of CHO cell pools using the piggyBac transposon system for mediating gene integration. The method describes the co-transfection of cells with the donor plasmid (coding for the gene of interest) and the helper plasmid (coding for the transposase) using polyethyleneimine (PEI). This is followed by a genetic selection for the generation of a cell pool. The resulting cell pool can be used to start a batch or fed-batch culture. Alternatively it can be used for generation of clonal cell lines or generation of cell banks for future use.

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Author information

Author notes

  1. Sowmya Balasubramanian

    Present address: ATUM, Newark, CA, USA

Authors and Affiliations

  1. Laboratory of Cellular Biotechnology (LBTC), École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland

    Sowmya Balasubramanian

Authors
  1. Sowmya Balasubramanian
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Editor information

Editors and Affiliations

  1. Protein Production and Structure Core Facility (PPSCF), Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland

    David L. Hacker

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Balasubramanian, S. (2018). Recombinant CHO Cell Pool Generation Using piggyBac Transposon System. In: Hacker, D. (eds) Recombinant Protein Expression in Mammalian Cells. Methods in Molecular Biology, vol 1850. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8730-6_6

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  • DOI: https://doi.org/10.1007/978-1-4939-8730-6_6

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8729-0

  • Online ISBN: 978-1-4939-8730-6

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