Abstract
Mast cells and basophils are important innate immune cells involved in resistance to parasitic infection and are critical orchestrators of allergic disease. The relative ease with which they are cultured from mouse or human tissues allows one to work with primary cells that maintain a differentiated and functional phenotype. In this chapter, we describe the methods by which mouse mast cells and basophils can be cultured from bone marrow. We also provide methods for isolating and expanding mouse peritoneal mast cells and human skin mast cells.
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Acknowledgments
This work was supported by the National Institutes of Health, grants 1R01AI101153 and 2R01AI059638 to J.J.R. and grants R21AR067996 and 1R01AI095494 to C.A.O.
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Caslin, H.L. et al. (2018). The Use of Human and Mouse Mast Cell and Basophil Cultures to Assess Type 2 Inflammation. In: Reinhardt, R. (eds) Type 2 Immunity. Methods in Molecular Biology, vol 1799. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-7896-0_8
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DOI: https://doi.org/10.1007/978-1-4939-7896-0_8
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