Abstract
Transmission electron microscopy (TEM) can provide excellent high-resolution images of cellular structures. A critical step with this technique is sample preparation. Here we describe our protocol for the preparation of semithin and ultrathin sections of mouse peripheral nerves in order to visualize the structure and pathological features of peripheral myelin with TEM.
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Acknowledgments
This work was supported by Fondazione Telethon (GGP14147) and the Italian Ministry of Health (GR-2011-0234679).
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Ferri, C., Quattrini, A., D’Antonio, M. (2018). Electron Microscopy for the Analysis of Peripheral Nerve Myelin. In: Woodhoo, A. (eds) Myelin. Methods in Molecular Biology, vol 1791. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7862-5_1
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DOI: https://doi.org/10.1007/978-1-4939-7862-5_1
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7861-8
Online ISBN: 978-1-4939-7862-5
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