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  • © 2018

Adipose-Derived Stem Cells

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1773)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xii
  2. A Method for Isolation of Stromal Vascular Fraction Cells in a Clinically Relevant Time Frame

    • Joel A. Aronowitz, Ryan A. Lockhart, Cloe S. Hakakian
    Pages 11-19
  3. Scaffold-Free, Size-Controlled Three-Dimensional Culture of Rabbit Adipose-Derived Stem Cells

    • Christina L. Rettinger, Kai P. Leung, Rodney K. Chan
    Pages 21-30
  4. Differentiation of Brown Adipocyte Progenitors Derived from Human Induced Pluripotent Stem Cells

    • Anne-Laure Hafner, Tala Mohsen-Kanson, Christian Dani
    Pages 31-39
  5. Methylcellulose Based Thermally Reversible Hydrogels

    • Anoosha Forghani, Ram Devireddy
    Pages 41-51
  6. Decellularized Adipose Tissue Scaffolds for Soft Tissue Regeneration and Adipose-Derived Stem/Stromal Cell Delivery

    • Pascal Morissette Martin, Arthi Shridhar, Claire Yu, Cody Brown, Lauren E. Flynn
    Pages 53-71
  7. Myogenic Differentiation of ASCs Using Biochemical and Biophysical Induction

    • Pinar Yilgor Huri, Justin Morrissette-McAlmon, Warren L. Grayson
    Pages 123-135
  8. Isolation of Murine Adipose-Derived Stromal/Stem Cells for Adipogenic Differentiation or Flow Cytometry-Based Analysis

    • Gail Kilroy, Marilyn Dietrich, Xiying Wu, Jeffrey M. Gimble, Z. Elizabeth Floyd
    Pages 137-146
  9. Three-Dimensional Magnetic Levitation Culture System Simulating White Adipose Tissue

    • Hubert Tseng, Alexes C. Daquinag, Glauco R. Souza, Mikhail G. Kolonin
    Pages 147-154
  10. Isolation of Human Adipose-Derived Stem Cells from Lipoaspirates

    • Jie Li, J. Lowry Curley, Z. Elizabeth Floyd, Xiying Wu, Yuan Di C. Halvorsen, Jeffrey M. Gimble
    Pages 155-165
  11. Soft Tissue Reconstruction

    • Francesco Egro, Kacey G. Marra
    Pages 203-213

About this book

This second edition volume expands on the previous edition with a comprehensive update on recent developments in the Adipose Stromal/Stem Cells (ASC) field and the use of stroma vascular fraction (SVF) as a potential therapeutic strategy. The chapters in this book discuss protocols that are focused on discovery, preclinical, and clinical processes. Although most chapters focus on human ASC, there are several chapters that look at small and large animal species with pre-clinical relevance. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips in troubleshooting and avoiding known pitfalls.



Cutting-edge and thorough, Adipose-Derived Stem Cells: Methods and Protocols, Second Edition is a valuable resource for anyone interested in learning more aboutthe scientific advances in the field of ASC biology and application.


Editors and Affiliations

  • Center for Stem Cell Research and Regenerative Medicine, Tulane University School of Medicine, New Orleans, USA

    Bruce A. Bunnell, Jeffrey M. Gimble

  • LaCell LLC, New Orleans, USA

    Jeffrey M. Gimble

Bibliographic Information

  • Book Title: Adipose-Derived Stem Cells

  • Book Subtitle: Methods and Protocols

  • Editors: Bruce A. Bunnell, Jeffrey M. Gimble

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7799-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-7797-0Published: 24 April 2018

  • Softcover ISBN: 978-1-4939-9288-1Published: 10 December 2019

  • eBook ISBN: 978-1-4939-7799-4Published: 23 April 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XII, 284

  • Number of Illustrations: 10 b/w illustrations, 46 illustrations in colour

  • Topics: Cell Biology

Buy it now

Buying options

eBook USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access