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Measuring Plant Root Traits Under Controlled and Field Conditions: Step-by-Step Procedures

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Root Development

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1761))

Abstract

In this chapter, we present methods that we routinely use to measure plant root traits in the field and under controlled environmental conditions (using rhizoboxes). We describe procedures to (1) collect, wash, and store root samples, (2) acquire images of washed root samples, and (3) measure root traits using image analysis. In addition, we also describe sampling methods for studying belowground productivity, soil exploration, and root distribution in the first soil layers at the community level (soil coring and ingrowth core method). Because the use of rhizoboxes allows a nondestructive and dynamic measurement of traits hardly accessible in the field, a section of this chapter is devoted to the acquisition and analysis of images of roots growing in rhizoboxes.

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Acknowledgments

We would like to thank Dr. Thomas Niemeyer (Leuphana University, Germany) for its excellent technical support. We are also thankful to Dr. Guillaume Lobet for providing some images used in this book chapter. This research was funded by CNPq Brazil (Ciência Sem Fronteiras Program) with the PhD scholarship of Emanuela W.A. Weidlich, BMBF in Germany for the INPLAMINT PhD project of Richard van Duijnen within the BonaRes soils programme, as well as by the Chair of Ecosystem Functioning and Services, Leuphana University, Lüneburg, Germany.

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Authors and Affiliations

  1. Ecosystem Functioning and Services, Institute of Ecology, Leuphana University, Lüneburg, Germany

    Benjamin M. Delory, Emanuela W. A. Weidlich, Richard van Duijnen & Vicky M. Temperton

  2. INRA, Centre PACA, UR 1115 PSH, Avignon Cedex 9, France

    Loïc Pagès

Authors
  1. Benjamin M. Delory
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  2. Emanuela W. A. Weidlich
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  3. Richard van Duijnen
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  4. Loïc Pagès
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  5. Vicky M. Temperton
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Corresponding author

Correspondence to Benjamin M. Delory .

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Editors and Affiliations

  1. Gregor Mendel Institute of Molecular Plant Biology, Vienna, Wien, Austria

    Daniela Ristova

  2. Institute of Applied Genetics & Cell Biology, University of Natural Resources and Life Sciences, Vienna, Wien, Austria

    Elke Barbez

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Delory, B.M., Weidlich, E.W.A., van Duijnen, R., Pagès, L., Temperton, V.M. (2018). Measuring Plant Root Traits Under Controlled and Field Conditions: Step-by-Step Procedures. In: Ristova, D., Barbez, E. (eds) Root Development. Methods in Molecular Biology, vol 1761. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7747-5_1

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  • DOI: https://doi.org/10.1007/978-1-4939-7747-5_1

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7746-8

  • Online ISBN: 978-1-4939-7747-5

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