Abstract
Hypoxia has long been recognized as a driving force of tumor progression and therapeutic resistance, and the transcription factor HIF-1α is believed to play a crucial role in these processes. Here we describe an efficient RCAS/Nes-TVA system that allows for in vivo manipulation of HIF-1α expression in the mouse neural progenitor cells. Simple production of the recombinant avian virus RCAS enables quick delivery of gene of interest through injection into the neural progenitors of transgenic mice expressing the viral cognate receptor TVA under the nestin promoter. By crossing with various commercially available genetically engineered mouse strains, a repertoire of mouse models can be created to study gene-specific effects on glioma genesis. This chapter provides details of plasmid construction, viral production, and intracranial delivery of transgenes, a methodology that can be easily adapted to a specific purpose.
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Acknowledgments
This work was supported in part by an NIH Grant CA084563 from the National Cancer Institute and by the University of Utah Funding Incentive Seed Grant. The authors wish to thank Kristin Kraus for editorial assistance.
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Tiburcio, P.D.B., Lyne, S.B., Eric Huang, L. (2018). In Vivo Manipulation of HIF-1α Expression During Glioma Genesis. In: Huang, L. (eds) Hypoxia. Methods in Molecular Biology, vol 1742. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7665-2_20
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DOI: https://doi.org/10.1007/978-1-4939-7665-2_20
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7664-5
Online ISBN: 978-1-4939-7665-2
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