Abstract
MicroRNAs (miRNAs), widely distributed, small regulatory RNA genes, target both messenger RNA (mRNA) degradation and suppression of protein translation based on sequence complementarity between the miRNA and its targeted mRNA. Different names have been used to describe various types of miRNA. During evolution, RNA retroviruses or transgenes invaded the eukaryotic genome and were inserted itself in the noncoding regions of DNA, conceivably acting as transposon-like jumping genes, providing defense from viral invasion and fine-tuning of gene expression as a secondary level of gene modulation in eukaryotes. When a transposon is inserted in the intron, it becomes an intronic miRNA, taking advantage of the protein synthesis machinery, i.e., mRNA transcription and splicing, as a means for processing and maturation. MiRNAs have been found to play an important, but not life-threatening, role in embryonic development. They might play a pivotal role in diverse biological systems in various organisms, facilitating a quick response and accurate plotting of body physiology and structures. Based on these unique properties, manufactured intronic miRNAs have been developed for in vitro evaluation of gene function, in vivo gene therapy, and generation of transgenic animal models. The biogenesis of miRNAs, circulating miRNAs, miRNAs and cancer, iPSCs, and heart disease are presented in this chapter, highlighting some recent studies on these topics.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Holley RW (1965) Structure of an alanine transfer ribonucleic acid. JAMA 194:868–871
Maxwell ES, Fournier MJ (1995) The small nucleolar RNAs. Annu Rev Biochem 64:897–934
Tycowski KT, Shu MD, Steitz JA (1996) A mammalian gene with introns instead of exons generating stable RNA products. Nature 379:464–466
Filipowicz W (2000) Imprinted expression of small nucleolar RNAs in brain: time for RNomics. Proc Natl Acad Sci U S A 97:14035–14037
Allmang C, Kufel J, Chanfreau G, Mitchell P, Petfalski E, Tollervey D (1999) Functions of the exosome in rRNA, snoRNA and snRNA synthesis. EMBO J 18:5399–5410
van Hoof A, Parker R (1999) The exosome: a proteasome for RNA? Cell 99:347–350
Frank DN, Roiha H, Guthrie C (1994) Architecture of the U5 small nuclear RNA. Mol Cell Biol 14:2180–2190
Stavianopoulos JG, Karkus JD, Charguff E (1971) Nucleic acid polymerase of the developing chicken embryos: a DNA Polymerase preferring a hybrid template. Proc Natl Acad Sci U S A 68:2207–2211
Stavianopoulos JG, Karkus JD, Charguff E (1972) Mechanism of DNA replication by highly purified DNA polymerase of chicken embryos. Proc Natl Acad Sci U S A 69:2609–2613
Wank H, Schroeder R (1996) Antibiotic-induced oligomerisation of group I intron RNA. J Mol Biol 258:53–61
van der Krol AR, Mur LA, Beld M, Mol JN, Stuitje AR (1990) Flavonoid genes in petunia: addition of a limited number of gene copies may lead to a suppression of gene expression. Plant Cell 2:291–299
Napoli C, Lemieux C, Jorgensen RA (1990) Introduction of a chimeric chalcone synthase gene into Petunia results in reversible co-suppression of homologous genes in trans. Plant Cell 2:279–289
Matzke MA, Primig MJ, Trnovsky J, Matzke AJM (1989) Reversible methylation and inactivation of marker genes in sequentially transformed tobacco plants. EMBO J 8:643–649
Fire A, Xu S, Montgomery MK, Kostas SA, Driver SE, Mello CC (1998) Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 391:806–811
Shi Y (2003) Mammalian RNAi for the masses. Trends Genet 19:9–12
Sui G, Soohoo C, Affar el B, Gay F, Shi Y, Forrester WC, Shi Y (2002) A DNA vector-based RNAi technology to suppress gene expression in mammalian cells. Proc Natl Acad Sci U S A 99:5515–5520
Elbashir SM, Lendeckel W, Tuschl T (2001) RNA interference is mediated by 21- and 22-nucleotide RNAs. Genes Dev 15:188–200
Pasquinelli AE, Reinhart BJ, Slack F, Martindale MQ, Kuroda MI, Maller B, Hayward DC, Ball EE, Degnan B, Müller P, Spring J, Srinivasan A, Fishman M, Finnerty J, Corbo J, Levine M, Leahy P, Davidson E, Ruvkun G (2000) Conservation of the sequence and temporal expression of let-7 heterochronic regulatory RNA. Nature 408:86–89
Reinhart BJ, Bartel DP (2002) Small RNAs correspond to centromere heterochromatic repeats. Science 297:1831
Kuwabara T, Hsieh J, Nakashima K, Taira K, Gage FH (2004) A small modulatory dsRNA specifies the fate of adult neural stem cells. Cell 116:779–793
Lee RC, Feinbaum RL, Ambros V (1993) The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14. Cell 75:843–854
Lau NC, Lim LP, Weinstein EG, Bartel DP (2001) An abundant class of tiny RNAs with probable regulatory roles in Caenorhabditis elegans. Science 294:858–862
Lagos-Quintana M, Rauhut R, Meyer J, Borkhardt A, Tuschl T (2003) New microRNAs from mouse and human. RNA 9:175–179
Lee Y, Ahn C, Han J, Choi H, Kim J, Yim J, Lee J, Provost P, Rådmark O, Kim S, Kim VN (2003) The nuclear RNase III Drosha initiates microRNA processing. Nature 425:415–419
Lund E, Guttinger S, Calado A, Dahlberg JE, Kutay U (2003) Nuclear export of microRNA precursors. Science 303:95–98
Ying SY, Lin SL (2005) Intronic microRNAs (miRNAs). Biochem Biophys Res Commun 326:515–520
Lee YS, Nakahara K, Pham JW, Kim K, He Z, Sontheimer EJ, Carthew RW (2004) Distinct roles for Drosophila Dicer-1 and Dicer-2 in the siRNA/miRNA silencing pathways. Cell 117:69–81
Tang G (2005) siRNA and miRNA: an insight into RISCs. Trends Biochem Sci 30:106–114
Lambowitz AM, Zimmerly S (2004) Mobile group II introns. Annu Rev Genet 38:1–35
Coghlan A, Wolfe KH (2004) Origins of recently gained introns in Caenorhabditis. Proc Natl Acad Sci U S A 101:11362–11367
Harper PS (1989) Myotonic dystrophy, 2nd edn. Saunders, London
Liquori CL, Ricker K, Moseley ML, Jacobsen JF, Kress W, Naylor SL, Day JW, Ranum LP (2001) Myotonic dystrophy type 2 caused by a CCTG expansion in intron 1 of ZNF9. Science 293:864–867
Aravin AA, Sachidanadam R, Girard A, Fejes-Toth K, Hannon GJ (2007) Developmentally regulated piRNA clusters implicate MILI in transposon control. Science 316:744–747
Siomi MC, Miyoshi T, Siomi H (2010) piRNA-mediated silencing in Drosophila germlines. Semin Cell Dev Biol 21:754–749
Betel D, Sheridan R, Marks DS, Sander C (2007) Computational analysis of mouse piRNA sequence and biogenesis. PLoS Comput Biol 3:2219–2227
Shpiz S, Kwon D, Rozovsky Y, Kalmykova A (2009) rasiRNA pathway controls antisense expression of Drosophila telomeric transposons in the nucleus. Nucleic Acids Res 37:267–278
Pelisson A, Sarot E, Payen-Groschene G, Bucheton A (2007) A novel repeat-associated small interfering RNA -mediated silencing pathway downregulates complementary sense gypsy transcripts in somatic cells of the Drosophila ovary. J Virol 81:1951–1960
Gasciolli V, Mallory AC, Bartel DP, Vaucheret H (2005) Partially redundant functions of Arabidopsis DICER-like enzymes and a role for DCL4 in producing trans-acting siRNAs. Curr Biol 15:1–7
Allen E, Xie Z, Gustafson AM, Carrington JC (2005) MicroRNA-directed phasing during trans-acting siRNA biogenesis in plants. Cell 121:207–221
Li LC, Okino ST, Zhao H, Pookot D, Place RF, Urakami S, Enokida H, Dahiya R (2006) Small dsRNAs induce transcriptional activation in human cells. Proc Natl Acad Sci U S A 103:17337–17342
Place RF, Li LC, Pookot D, Noonan EJ, Dahiya R (2008) MicroRNA-373 induces expression of genes with complementary promoter sequences. Proc Natl Acad Sci U S A 105:1608–1613
Zhang H, Zhu JK (2014) Emerging roles of RNA processing factors in regulating long non-coding RNAs. RNA Biol 11:793–797
Batista PJ, Chang HY (2013) Long noncoding RNAs: cellular address codes in development and disease. Cell 152:1298–1307
Lee JT, Bartolomei MS (2013) X-Inactivation, imprinting, and long noncoding RNAs in health and disease. Cell 152:1308–1323
Chen LL, Carmichael GG (2010) Decoding the function of nuclear long non-coding RNAs. Curr Opin Cell Biol 22:357–364
Altuvia Y, Landgraf P, Lithwick G, Elefant N, Pfeffer S, Aravin A, Brownstein MJ, Tuschl T, Margalit H (2005) Clustering and conservation patterns of human microRNAs. Nucleic Acids Res 33:2697–2706
Lee Y, Kim M, Han J, Yeom KH, Lee S, Baek SH, Kim VN (2004) MicroRNA genes are transcribed by RNA polymerase II. EMBO J 23:4051–4060
Borchert GM, Lanier W, Davidson BL (2006) RNA polymerase III transcribes human microRNAs. Nat Struct Mol Biol 13:1097–1101
Lin SL, Chang D, DY W, Ying SY (2003) A novel RNA splicing-mediated gene silencing mechanism potential for genome evolution. Biochem Biophys Res Commun 310:754–760
Yi R, Qin Y, Macara IG, Cullen BR (2003) Exportin-5 mediates the nuclear export of pre-microRNAs and short hairpin RNAs. Genes Dev 17:3011–3016
Meijer HA, Smith EM, Bushell M (2014) Regulation of miRNA strand selection: follow the leader? Biochem Soc Trans 42:1135–1140
Khvorova A, Reynolds A, Jayasena SD (2003) Functional siRNAs and miRNAs exhibit strand bias. Cell 115:209–216
Hammond SM (2014) An overview of microRNAs. Nat Rev Mol Cell Biol 15:509–524
Ha M, Kim VN (2015) Regulation of microRNA biogenesis. Adv Drug Deliv Rev 87:3–14
Yang JS, Lai EC (2011) Alternative miRNA biogenesis pathways and the interpretation of core miRNA pathway mutants. Mol Cell 43:892–903
Daugaard I, Hansen TB (2017) Biogenesis and function of Ago-associated RNAs. Trends Genet 33:208–219
Watson JD (2008) Molecular biology of the gene. Cold Spring Harbor Laboratory Press, San Francisco, CA, pp 641–648
Dueck A, Meister G (2014) Assembly and function of small RNA - argonaute protein complexes. Biol Chem 395:611–629
Meister G (2013) Argonaute proteins: functional insights and emerging roles. Nat Rev Genet 14:447–459
Brennecke J et al (2005) Principles of microRNA-target recognition. PLoS Biol 3:e85
Doench JG, Sharp PA (2004) Specificity of microRNA target selection in translational repression. Genes Dev 18:504–511
Lai EC (2002) microRNAs are complementary to 3′ UTR sequence motifs that mediate negative post-transcriptional regulation. Nat Genet 30:363–364
Jee D, Lai EC (2014) Alteration of miRNA activity via context-specific modifications of Argonaute proteins. Trends Cell Biol 24:546–553
Nakanishi K (2016) Anatomy of RISC: how do small RNAs and chaperones activate Argonaute proteins? Wiley Interdiscip Rev RNA 7:637–660
Ana Eulalio A, Felix Tritschler F, Regina Büttner R, Oliver Weichenrieder O, Elisa Izaurralde E, Vincent Truffault V (2009) The RRM domain in GW182 proteins contributes to miRNA-mediated gene silencing. Nucleic Acids Res 37:2974–2983
Wilson RC, Tambe A, Kidwell MA, Noland CL, Schneider CP, Doudna JA (2015) Dicer-TRBP complex formation ensures accurate mammalian microRNA biogenesis. Mol Cell 57:397–407
Grosshans H (2010) Regulation of MicroRNAs. Springer Science & Business Media, New York, NY
Cho CJ, Myung SJ, Chang S (2017) ADAR1 and microRNA; a hidden crosstalk in cancer. Int J Mol Sci 18(4):pii: E799. https://doi.org/10.3390/ijms18040799
Skeparnias I, Αnastasakis D, Shaukat AN, Grafanaki K, Stathopoulos C (2015) Expanding the repertoire of deadenylases. RNA Biol 7:1–6
Zhang X, Devany E, Murphy MR, Glazman G, Persaud M, Kleiman FE (2015) PARN deadenylase is involved in miRNA-dependent degradation of TP53 mRNA in mammalian cells. Nucleic Acids Res 43:10925–10938
Svobodova E, Kubikova J, Svoboda P (2016) Production of small RNAs by mammalian Dicer. Pflugers Arch 468:1089–1102
Fitzgerald ME, Vela A, Pyle AM (2014) Dicer-related helicase 3 forms an obligate dimer for recognizing 22G-RNA. Nucleic Acids Res 42:3919–3930
Yi T, Arthanari H, Akabayov B, Song H, Papadopoulos E, Qi HH, Jedrychowski M, Güttler T, Guo C, Luna RE, Gygi SP, Huang SA, Wagner G (2015) eIF1A augments Ago2-mediated Dicer-independent miRNA biogenesis and RNA interference. Nat Commun 6:7194. https://doi.org/10.1038/ncomms8194
Pillai RS, Artus CG, Filipowicz W (2004) Tethering of human Ago proteins to mRNA mimics the miRNA-mediated repression of protein synthesis. RNA 10:1518–1525
Richard WC, Erik JS (2009) Origins and mechanisms of miRNAs and siRNAs. Cell 136:642–655
Fabbri M, Paone A, Calore F, Galli R, Gaudio E, Santhanam R, Lovat F, Fadda P, Mao C, Nuovo GJ, Zanesi N, Crawford M, Ozer GH, Wernicke D, Alder H, Caligiuri MA, Nana-Sinkam P, Perrotti D, Croce CM (2012) MicroRNAs bind to Toll-like receptors to induce prometastatic inflammatory response. Proc Natl Acad Sci U S A 109:E2110–E2116
He S, Chu J, Wu LC, Mao H, Peng Y, Alvarez-Breckenridge CA, Hughes T, Wei M, Zhang J, Yuan S, Sandhu S, Vasu S, Benson DM Jr, Hofmeister CC, He X, Ghoshal K, Devine SM, Caligiuri MA, Yu J (2013) MicroRNAs activate natural killer cells through Toll-like receptor signaling. Blood 121:4663–4671
Hessvik NP, Sandvig K, Llorente A (2013) Exosomal miRNAs as biomarkers for prostate cancer. Front Genet 1819:1154–1163
Phuyal S, Hessvik NP, Skotland T, Sandvig K, Llorente A (2014) Regulation of exosome release by glycosphingolipids and flotillins. FEBS J 281:2214–2227
Marfella R, Di Filippo C, Potenza N, Sardu C, Rizzo MR, Siniscalchi M, Musacchio E, Barbieri M, Mauro C, Mosca N, Solimene F, Mottola MT, Russo A, Rossi F, Paolisso G, D’Amico M (2013) Circulating microRNA changes in heart failure patients treated with cardiac resynchronization therapy: responders vs. non-responders. Eur J Heart Fail 15:1277–1288
Raposo G, Stoorvogel W (2013) Extracellular vesicles: exosomes, microvesicles, and friends. J Cell Biol 200:373–383
Harding CV, Heuser JE, Stahl PD (2013) Exosomes: looking back three decades and into the future. J Cell Niol 200:367–371
Cortez MA, Bueso-Ramos C, Ferdin J, Lopez-Berestein G, Sood AK, Calin GA (2011) MicroRNAs in body fluids--the mix of hormones and biomarkers. Nat Rev Clin Oncol 8:467–477
Creemers EE, Tijsen AJ, Pinto YM (2012) Circulating microRNAs: novel biomarkers and extracellular communicators in cardiovascular disease? Circ Res 110:483–495
Etheridge A, Lee I, Hood L, Galas D, Wang K (2017) Extracellular microRNA: a new source of biomarkers. Mutat Res 717:85–90
Lin SL, Chang DC, Chang-Lin S, Lin CH, DT W, Chen DT, Ying SY (2008) Mir-302 reprograms human skin cancer cells into a pluripotent ES-cell-like state. RNA 14:2115–2124
Lin SL, Chang DC, Ying SY, Leu D, Wu DT (2010) MicroRNA miR-302 inhibits the tumorigenecity of human pluripotent stem cells by coordinate suppression of the CDK2 and CDK4/6 cell cycle pathways. Cancer Res 70:9473–9482
Tian Y, Liu Y, Wang T, Zhou N, Kong J, Chen L, Snitow M, Morley M, Li D, Petrenko N, Zhou S, Lu M, Gao E, Koch WJ, Stewart KM, Morrisey EE (2015) A microRNA-Hippo pathway that promotes cardiomyocyte proliferation and cardiac regeneration in mice. Sci Transl Med 7(279):279ra38. https://doi.org/10.1126/scitranslmed.3010841
Liu M, Tao G, Liu Q, Liu K, Yang X (2017) MicroRNA let-7g alleviates atherosclerosis via the targeting of LOX-1 in vitro and in vivo. Int J Mol Med 40:57. https://doi.org/10.3892/ijmm.2017.2995
Chang-Lin S, Hung A, Chang DC, Lin YW, Ying SY, Lin SL (2016) Novel glycylated sugar alcohols protect ESC-specific microRNAs from degradation in iPS cells. Nucleic Acids Res 44:4894–4906
Huang X, Jia Z (2013) Construction of HCC-targeting artificial miRNAs using natural miRNA precursors. Exp Ther Med 6:209–215
Bartel D (2004) MicroRNAs: genomics, biogenesis, mechanism, and function. Cell 116:281–297
Zhang B, Pan X, Cobb GP, Anderson TA (2007) microRNAs as oncogenes and tumor suppressors. Dev Biol 302:1–12
Baranwal S, Alahari SK (2010) miRNA control of tumor cell invasion and metastasis. Int J Cancer 126:1283–1222
Palma Flores C, GarcÃa-Vázquez R, Gallardo Rincón D, Ruiz-GarcÃa E, Astudillo de la Vega H, Marchat LA, Salinas Vera YM, López-Camarillo C (2017) MicroRNAs driving invasion and metastasis in ovarian cancer: Opportunities for translational medicine. Int J Oncol 50:1461–1476
Yan J, Ma C, Gao Y (2017) MicroRNA-30a-5p suppresses epithelial-mesenchymal transition by targeting profilin-2 in high invasive non-small cell lung cancer cell lines. Oncol Rep 37:3146–3154
Jansson MD, Lund AH (2012) MicroRNA and cancer. Mol Oncol 6:590–610
Farazi TA, Hoell JI, Morozov P, Tuschl T (2013) MicroRNAs in human cancer. Adv Exp Med Biol 774:1–20
Acunzo M, Romano G, Wernicke D, Croce CM (2015) MicroRNA and cancer--a brief overview. Adv Biol Regul 57:1–9
Iorio MV, Croce CM (2017) MicroRNA dysregulation in cancer: diagnostics, monitoring and therapeutics. A comprehensive review. EMBO Mol Med 4:143. 10.15252/emmm.201707779
Rupaimoole R, Slack FJ (2017) MicroRNA therapeutics: towards a new era for the management of cancer and other diseases. Nat Rev Drug Discov 16:203–222
Suh MR, Lee Y, Kim JY, Kim SK, Moon SH, Lee JY, Cha KY, Chung HM, Yoon HS, Moon SY, Kim VN, Kim KS (2004) Human embryonic stem cells express a unique set of microRNAs. Dev Biol 270:488–498
Li HL, Wei JF, Fan LY, Wang SH, Zhu L, Li TP, Lin G, Sun Y, Sun ZJ, Ding J, Liang XL, Li J, Han Q, Zhao RC (2016) miR-302 regulates pluripotency, teratoma formation and differentiation in stem cells via an AKT1/OCT4-dependent manner. Cell Death Dis 7:e2078. https://doi.org/10.1038/cddis.2015.383
Liang Z, Ahn J, Guo D, Votaw JR, Shim H (2013) MicroRNA-302 replacement therapy sensitizes breast cancer cells to ionizing radiation. Pharm Res 30:1008–1016
Wang Y, Zhao L, Xiao Q, Jiang L, He M, Bai X, Ma M, Jiao X, Wei M (2016) miR-302a/b/c/d cooperatively inhibit BCRP expression to increase drug sensitivity in breast cancer cells. Gynecol Oncol 141:592–601
Zhao L, Wang Y, Jiang L, He M, Bai X, Yu L, Wei M (2016) MiR-302a/b/c/d cooperatively sensitizes breast cancer cells to adriamycin via suppressing P-glycoprotein (P-gp) by targeting MAP/ERK kinase kinase 1 (MEKK1). J Exp Clin Cancer Res 35:25. https://doi.org/10.1186/s13046-016-0300-8
Ge T, Yin M, Yang M, Liu T, Lou G (2014) MicroRNA-302b suppresses human epithelial ovarian cancer cell growth by targeting RUNX1. Cell Physiol Biochem 34:2209–2220
Yan GJ, Yu F, Wang B, Zhou HJ, Ge QY, Su J, YL H, Sun HX, Ding LJ (2014) MicroRNA miR-302 inhibits the tumorigenicity of endometrial cancer cells by suppression of Cyclin D1 and CDK1. Cancer Lett 345:39–47
Cai N, Wang YD, Zheng PS (2013) The microRNA-302-367 cluster suppresses the proliferation of cervical carcinoma cells through the novel target AKT1. RNA 19:85–95
Maadi H, Moshtaghian A, Taha MF, Mowla SJ, Kazeroonian A, Haass NK, Javeri A (2016) Multimodal tumor suppression by miR-302 cluster in melanoma and colon cancer. Int J Biochem Cell Biol 81(Pt A):121–132
Wang L, Yao J, Shi X, Hu L, Li Z, Song T, Huang C (2013) MicroRNA-302b suppresses cell proliferation by targeting EGFR in human hepatocellular carcinoma SMMC-7721 cells. BMC Cancer 13:448. https://doi.org/10.1186/1471-2407-13-448
Koga C, Kobayashi S, Nagano H, Tomimaru Y, Hama N, Wada H, Kawamoto K, Eguchi H, Konno M, Ishii H, Umeshita K, Doki Y, Mori M (2014) Reprogramming using microRNA-302 improves drug sensitivity in hepatocellular carcinoma cells. Ann Surg Oncol Suppl 4:S591–S600
Wang L, Yao J, Zhang X, Guo B, Le X, Cubberly M, Li Z, Nan K, Song T, Huang C (2014) miRNA-302b suppresses human hepatocellular carcinoma by targeting AKT2. Mol Cancer Res 12:190–202
Cai D, He K, Chang S, Tong D, Huang C (2015) MicroRNA-302b enhances the sensitivity of hepatocellular carcinoma cell lines to 5-FU via targeting Mcl-1 and DPYD. Int J Mol Sci 16:23668–23682
Bourguignon LY, Wong G, Earle C, Chen L (2012) Hyaluronan-CD44v3 interaction with Oct4-Sox2-Nanog promotes miR-302 expression leading to self-renewal, clonal formation, and cisplatin resistance in cancer stem cells from head and neck squamous cell carcinoma. J Biol Chem 287:32800–12824
Chen L, Min L, Wang X, Zhao J, Chen H, Qin J, Chen W, Shen Z, Tang Z, Gan Q, Ruan Y, Sun Y, Qin X, Gu J (2015) Loss of RACK1 promotes metastasis of gastric cancer by inducing a miR-302c/IL8 signaling loop. Cancer Res 75:3832–3841
Khodayari N, Mohammed KA, Lee H, Kaye F, Nasreen N (2016) MicroRNA-302b targets Mcl-1 and inhibits cell proliferation and induces apoptosis in malignant pleural mesothelioma cells. Am J Cancer Res 6:1996–2009
Hu Q, Wang YB, Zeng P, Yan GQ, Xin L, Hu XY (2017) Expression of long non-coding RNA (lncRNA) H19 in immunodeficient mice induced with human colon cancer cells. Eur Rev Med Pharmacol Sci 20:4880–4884
Ji Q, Liu X, Fu X, Zhang L, Sui H, Zhou L, Sun J, Cai J, Qin J, Ren J, Li Q (2014) Resveratrol inhibits invasion and metastasis of colorectal cancer cells via MALAT1 mediated Wnt/β-catenin signal pathway. PLoS One 8:e78700. https://doi.org/10.1371/journal.pone.0078700
Ma T, Wang RP, Zou X (2016) Dioscin inhibits gastric tumor growth through regulating the expression level of lncRNA HOTAIR. BMC Complement Altern Med 16(1):383
Li SP, HX X, Yu Y, He JD, Wang Z, YJ X, Wang CY, Zhang HM, Zhang RX, Zhang JJ, Yao Z, Shen ZY (2016) LncRNA HULC enhances epithelial-mesenchymal transition to promote tumorigenesis and metastasis of hepatocellular carcinoma via the miR-200a-3p/ZEB1 signaling pathway. Oncotarget 7:42431–42446
Li W, Li H, Zhang L, Hu M, Li F, Deng J, An M, Wu S, Ma R, Lu J, Zhou Y (2017) Long non-coding RNA LINC00672 contributes to p53 protein-mediated gene suppression and promotes endometrial cancer chemosensitivity. J Biol Chem 292:5801–5813
Zhang Z, Zhou C, Chang Y, Zhang Z, Hu Y, Zhang F, Lu Y, Zheng L, Zhang W, Li X, Li X (2016) Long non-coding RNA CASC11 interacts with hnRNP-K and activates the WNT/β-catenin pathway to promote growth and metastasis in colorectal cancer. Cancer Lett 376:62–73
Nagini S (2017) Breast cancer: current molecular therapeutic targets and new players. Anticancer Agents Med Chem 17:152–163
Li AX, Xin WQ, Ma CG (2015) Fentanyl inhibits the invasion and migration of colorectal cancer cells via inhibiting the negative regulation of Ets-1 on BANCR. Biochem Biophys Res Commun 465:594–600
Zhou X, Ji G, Ke X, Gu H, Jin W, Zhang G (2015) MiR-141 inhibits gastric cancer proliferation by interacting with long noncoding RNA MEG3 and down-regulating E2F3 expression. Dig Dis Sci 60:3271–3282
Guo Q, Cheng Y, Liang T, He Y, Ren C, Sun L, Zhang G (2015) Comprehensive analysis of lncRNA-mRNA co-expression patterns identifies immune-associated lncRNA biomarkers in ovarian cancer malignant progression. Sci Rep 5:17683. https://doi.org/10.1038/srep17683
Chang L, Li C, Lan T, Wu L, Yuan Y, Liu Q, Liu Z (2016) Decreased expression of long non-coding RNA GAS5 indicates a poor prognosis and promotes cell proliferation and invasion in hepatocellular carcinoma by regulating vimentin. Mol Med Rep 13:1541–1550
Xue Y, Ni T, Jiang Y, Li Y (2017) LncRNA GAS5 inhibits tumorigenesis and enhances radiosensitivity by suppressing miR-135b expression in non-small cell lung cancer. Oncol Res 25:1305. https://doi.org/10.3727/096504017X14850182723737
Mei Y, Si J, Wang Y, Huang Z, Zhu H, Feng S, Wu X, Wu L (2017) Long noncoding RNA GAS5 suppresses tumorigenesis by inhibiting miR-23a 5 expression in non-small cell lung cancer. Oncol Res 25:1027. https://doi.org/10.3727/096504016X14822800040451
Judson RL, Babiarz JE, Venere M, Blelloch R (2009) Embryonic stem cell-specific microRNAs promote induced pluripotency. Nat Biotechnol 27:459–461
Li N, Long B, Han W, Yuan S, Wang K (2017) microRNAs: important regulators of stem cells. Stem Cell Res Ther 8:110. https://doi.org/10.1186/s13287-017-0551-0
Rosa A, Brivanlou AH (2013) Regulatory non-coding RNAs in pluripotent stem cells. Int J Mol Sci 14:14346–14373
Stadler B, Ivanovska I, Mehta K, Song S, Nelson A, Tan Y, Mathieu J, Darby C, Blau CA, Ware C, Peters G, Miller DG, Shen L, Cleary MA, Ruohola-Baker H (2010) Characterization of microRNAs involved in embryonic stem cell states. Stem Cells Dev 19:935–950
Lin SL, Chang DC, Lin CH, Ying SY, Leu D, Wu DT (2011) Regulation of somatic cell reprogramming through inducible mir-302 expression. Nucleic Acids Res 39:1054–1065, 2011
Kuo CH, Ying SY (2012) Advances in microRNA-mediated reprogramming technology. Stem Cells Int 2012:823709. https://doi.org/10.1155/2012/823709
Slack JM (2009) Metaplasia and somatic cell reprogramming. J Pathol 217:161–168
Li W, Nakanishi M, Zumsteg A, Shear M, Wright C, Melton DA, Zhou Q (2014) In vivo reprogramming of pancreatic acinar cells to three islet endocrine subtypes. Elife 3:e01846. https://doi.org/10.7554/eLife.01846
Anokye-Danso F, Trivedi CM, Juhr D, Gupta M, Cui Z, Tian Y, Zhang Y, Yang W, Gruber PJ, Epstein JA, Morrisey EE (2011) Highly efficient miRNA-mediated reprogramming of mouse and human somatic cells to pluripotency. Cell Stem Cell 8:376–388
Miyoshi N, Ishii H, Nagano H, Haraguchi N, Dewi DL, Kano Y, Nishikawa S, Tanemura M, Mimori K, Tanaka F, Saito T, Nishimura J, Takemasa I, Mizushima T, Ikeda M, Yamamoto H, Sekimoto M, Doki Y, Mori M (2011 Jun 3) (2011) Reprogramming of mouse and human cells to pluripotency using mature microRNAs. Cell Stem Cell 8(6):633–638. https://doi.org/10.1016/j.stem.2011.05.001
Barroso-delJesus A, Lucena-Aguilar G, Sanchez L, Ligero G, Gutierrez-Aranda I, Menendez P (2011) The Nodal inhibitor Lefty is negatively modulated by the microRNA miR-302 in human embryonic stem cells. FASEB J 25:1497–1508
Liao B, Bao X, Liu L, Feng S, Zovoilis A, Liu W, Xue Y, Cai J, Guo X, Qin B, Zhang R, Wu J, Lai L, Teng M, Niu L, Zhang B, Esteban MA, Pei D (2011) MicroRNA cluster 302–367 enhances somatic cell reprogramming by accelerating a mesenchymal-to-epithelial transition. J Biol Chem 286:17359–17364
Subramanyam D, Lamouille S, Judson RL, Liu JY, Bucay N, Derynck R, Blelloch R (2011) Multiple targets of miR-302 and miR-372 promote reprogramming of human fibroblasts to induced pluripotent stem cells. Nat Biotechnol 29:443–448
Lipchina I, Elkabetz Y, Hafner M, Sheridan R, Mihailovic A, Tuchil T, Sander C, Studer L, Betel D (2011) Genome-wide identification of microRNA targets human ES cells reveals a role for miR-302 in modulating BMP response. Genes Dev 25:2173–2186
Liu H, Deng S, Zhao Z, Zhang H, Xiao J, Song W, Gao F, Guan Y (2011) Oct4 regulates the miR-302 cluster in P19 mouse embryonic carcinoma cells. Mol Biol Rep 38:2155–2160
Card DA, Hebbar PB, Li L, Trotter KW, Komatsu Y, Mishina Y, Archer TK (2008) Oct4/Sox2-regulated miR-302 targets cyclin D1 in human embryonic stem cells. Mol Cell Biol 28:6426–6438
Hu S, Wilson KD, Ghosh Z, Han L, Wang Y, Lan F, Ransohoff KJ, Burridge P, Wu JC (2013) MicroRNA-302 increases reprogramming efficiency via repression of NR2F2. Stem Cells 31:259–268
Lin SL (2011) Concise review: deciphering the mechanism behind induced pluripotent stem cell generation. Stem Cells 29:1645–1649
Terasawa K, Shimizu K, Tsujimoto G (2011) Synthetic pre-miRNA-based shRNA as potent RNAi triggers. J Nucleic Acids 2011:131579
Gurha P (2016) MicroRNAs in cardiovascular disease. Curr Opin Cardiol 31:249–254
van Rooij E, Olson EN (2012) MicroRNA therapeutics for cardiovascular disease: opportunities and obstacles. Nat Rev Drug Discov 11:860–872
van Amerongen MJ, Felix B, Engel FB (2008) Features of cardiomyocyte proliferation and its potential for cardiac regeneration. J Cell Mol Med 12:2233–2244
Wu G, Huang ZP, Wang DZ (2013) MicroRNAs in cardiac regeneration and cardiovascular disease. Sci China Life Sci 56:907–913
Boon RA, Iekushi K, Lechner S, Seeger T, Fischer A, Heydt S, Kaluza D, Tréguer K, Carmona G, Bonauer A, Horrevoets AJ, Didier N, Girmatsion Z, Biliczki P, Ehrlich JR, Katus HA, Müller OJ, Potente M, Zeiher AM, Hermeking H, Dimmeler S (2013) MicroRNA-34a regulates cardiac ageing and function. Nature 495:107–110
Porrello ER, Johnson BA, Aurora AB, Simpson E, Nam YJ, Matkovich SJ, Dorn GW II, van Rooij E, Olson EN (2011) MiR-15 family regulates postnatal mitotic arrest of cardiomyocytes. Circ Res 109:670–679
Porrello ER, Mahmoud AI, Simpson E, Johnson BA, Grinsfelder D, Canseco D, Mammen PP, Rothermel BA, Olson EN, Sadek HA (2013) Regulation of neonatal and adult mammalian heart regeneration by the miR-15 family. Proc Natl Acad Sci U S A 110:187–192
Eulalio A, Mano M, Dal Ferro M, Zentilin L, Sinagra G, Zacchigna S, Giacca M (2012) Functional screening identifies miRNAs inducing cardiac regeneration. Nature 492:376–381
Ikeda S, He A, Kong SW, Lu J, Bejar R, Bodyak N, Lee KH, Ma Q, Kang PM, Golub TR, Pu WT (2009) MicroRNA‑1 negatively regulates expression of the hypertrophy-associated calmodulin and Mef2a genes. Mol. Cell. Biol 29:2193–2204
Montgomery RL, Hullinger TG, Semus HM, Dickinson BA, Seto AG, Lynch JM, Stack C, Latimer PA, Olsen EN, van Rooij E (2011) Therapeutic inhibition of miR‑208a improves cardiac function and survival during heart failure. Circulation 124:1537–1547
Sluijter JP, van Mil A, van Vliet P, Metz CH, Liu J, Doevendans PA, Goumans MJ (2010) MicroRNA-1 and -499 regulate differentiation and proliferation in human-derived cardiomyocyte progenitor cells. Arterioscler Thromb Vasc Biol 30:859–868
Yoo JK, Kim J, Choi SJ, Noh HM, Kwon YD, Yoo H, Yi HS, Chung HM, Kim JK (2012) Discovery and characterization of novel microRNAs during endothelial differentiation of human embryonic stem cells. Stem Cells Dev 21:2049–2057
Zhao W, Zhao SP, Zhao YH (2015) MicroRNA-143/-145 in cardiovascular diseases. Biomed Res Int 2015:531740
Chen T, Margariti A, Kelaini S, Cochrane A, Guha ST, Hu Y, Stitt AW, Zhang L, Xu Q (2015) MicroRNA-199b modulates vascular cell fate during iPS cell differentiation by targeting the Notch ligand Jagged1 and enhancing VEGF signaling. Stem Cells 33:1405–1418
Montgomery RL, Yu G, Latimer PA, Stack C, Robinson K, Dalby CM, Kaminski N, van Rooij E (2014) MicroRNA mimicry blocks pulmonary fibrosis. EMBO Mol Med 6:1347–1356
Cheng AM, Byrom MW, Jeffrey Shelton J, Lance P, Ford LP (2005) Antisense inhibition of human miRNAs and indications for an involvement of miRNA in cell growth and apoptosis. Nucleic Acids Res 33:1290–1297
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2018 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Ying, SY., Chang, D.C., Lin, SL. (2018). The MicroRNA. In: Ying, SY. (eds) MicroRNA Protocols . Methods in Molecular Biology, vol 1733. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7601-0_1
Download citation
DOI: https://doi.org/10.1007/978-1-4939-7601-0_1
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7600-3
Online ISBN: 978-1-4939-7601-0
eBook Packages: Springer Protocols