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AMPK

Methods and Protocols

  • Dietbert Neumann
  • Benoit Viollet

Part of the Methods in Molecular Biology book series (MIMB, volume 1732)

Table of contents

  1. Front Matter
    Pages i-xvii
  2. Christopher G. Langendorf, Jonathan S. Oakhill, Bruce E. Kemp
    Pages 15-27
  3. Ravi G. Kurumbail, Graham M. West, Venkatasubramanian Dharmarajan, Kris A. Borzilleri, Jane M. Withka, Jessica Ward et al.
    Pages 29-55
  4. Fiona A. Fyffe, Simon A. Hawley, Alexander Gray, D. Grahame Hardie
    Pages 69-86
  5. Brendan Gongol, Traci Marin, David A. Johnson, John Y.-J. Shyy
    Pages 99-109
  6. Arpit Jain, Valentin Roustan, Wolfram Weckwerth, Ingo Ebersberger
    Pages 111-142
  7. Pascual Sanz, Rosa Viana, Maria Adelaida Garcia-Gimeno
    Pages 143-157
  8. Jonathan S. Oakhill, John W. Scott, Toby A. Dite
    Pages 159-169
  9. Adrien Grenier, Pierre Sujobert, Séverine Olivier, Hélène Guermouche, Johanna Mondésir, Olivier Kosmider et al.
    Pages 171-194
  10. Biplab Dasgupta, William Seibel
    Pages 195-202
  11. Simon A. Hawley, Fiona A. Fyffe, Fiona M. Russell, Graeme J. Gowans, D. Grahame Hardie
    Pages 239-253
  12. Takafumi Miyamoto, Elmer Rho, Allen Kim, Takanari Inoue
    Pages 255-272
  13. Claudia Marinangeli, Jérome Kluza, Philippe Marchetti, Luc Buée, Valérie Vingtdeux
    Pages 289-305

About this book

Introduction

This volume explores the latest methods used to study AMPK by computational, biochemical, biophysical, cellular, and ex vivo and in vivo approaches. The chapters in this book cover specific topics, such as methods to measure change in cellular energy metabolism and analyze metabolic pathways regulated by AMPK; bioinformatics tools to identify AMPK targets; knockdown of AMPK by CRISPR-Cas9; production and crystallization of full-length human AMP-activated protein kinase; cell-free assays to measure the effects of regulatory ligands on AMPK; use of sensors of AMPK activity; AMPK protein interaction by yeast two-hybrid; the role of AMPK in inflammation and autophagy; analyzing the AMPK function in C. elegans and mammals (with special focus on skeletal muscle, blood vessels, kidneys, pancreatic islets and hypothalamus); and human γ2 AMPK mutations. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting edge and thorough, AMPK: Methods and Protocols is a valuable resource for graduate students, postdoctoral researchers, and established investigators who are interested in the richly diverse AMPK field.

Keywords

bacterial expression baculoviral upstream kinase protein crystallisation pharmacological activators

Editors and affiliations

  • Dietbert Neumann
    • 1
  • Benoit Viollet
    • 2
  1. 1.Department of Pathology CARIM School for Cardiovascular DiseasesMaastricht UniversityMaastrichtThe Netherlands
  2. 2.Department EMD, Inserm, U1016, Institut Cochin, CNRS, UMR8104Université Paris DescartesParisFrance

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-7598-3
  • Copyright Information Springer Science+Business Media, LLC 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7597-6
  • Online ISBN 978-1-4939-7598-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site