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NaV Channels: Assaying Biosynthesis, Trafficking, Function

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The Surfaceome

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1722))

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Abstract

Integral to the cell surface is channels, pumps, and exchanger proteins that facilitate the movement of ions across the membrane. Ion channels facilitate the passive movement of ions down an electrochemical gradient. Ion pumps actively use energy to actively translocate ions, often against concentration or voltage gradients, while ion exchangers utilize energy to couple the transport of different ion species such that one ion moves down its gradient and the released free energy is used to drive the movement of a different ion against its electrochemical gradient. Some ion pumps and exchangers may be electrogenic, i.e., the ion transport they support is not electrically neutral and generates a current. Functions of these pore-forming membrane proteins include the establishment of membrane potentials, gating of ions flows across the cell membrane to elicit action potentials and other electrical signals, as well as the regulation of cell volumes. The major forms of ion channels include voltage-, ligand-, and signal-gated channels. In this review, we describe mammalian voltage dependent Na (NaV) channels.

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Authors and Affiliations

  1. Division of Cardiology, Department of Medicine, Johns Hopkins University, Baltimore, MD, USA

    Gordon F. Tomaselli & Federica Farinelli

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  1. Gordon F. Tomaselli
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  2. Federica Farinelli
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Correspondence to Gordon F. Tomaselli .

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  1. School of Biomedical Sciences, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong, SAR, China

    Kenneth R. Boheler

  2. Department of Biochemistry, Medical College of Wisconsin, Milwaukee, Wisconsin, USA

    Rebekah L. Gundry

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Tomaselli, G.F., Farinelli, F. (2018). NaV Channels: Assaying Biosynthesis, Trafficking, Function. In: Boheler, K., Gundry, R. (eds) The Surfaceome. Methods in Molecular Biology, vol 1722. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7553-2_11

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  • DOI: https://doi.org/10.1007/978-1-4939-7553-2_11

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