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The Surfaceome

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Kenneth R. Boheler

    1. School of Biomedical Sciences, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong, SAR, China

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    You can also search for this editor in PubMed   Google Scholar

  2. Rebekah L. Gundry

    1. Department of Biochemistry, Medical College of Wisconsin, Milwaukee, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1722)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Discovery-Based Approaches to Surfaceome Content

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Surfaceome Analysis Protocol for the Identification of Novel Bordetella pertussis Antigens

      • Yulanda M. Williamson, Jennifer Whitmon, Rolieria West-Deadwyler, Hercules Moura, Adrian R. Woolfitt, Jon Rees et al.
      Pages 3-20

    3. “Shaving” Live Bacterial Cells with Proteases for Proteomic Analysis of Surface Proteins

      • Manuel J. Rodríguez-Ortega
      Pages 21-29

    4. Methods for Mapping the Extracellular and Membrane Proteome in the Avian Embryo, and Identification of Putative Vascular Targets or Endothelial Genes

      • Witold W. Kilarski, John Herbert, Andreas Bikfalvi
      Pages 31-56

    5. Mass Spectrometry-Based Identification of Extracellular Domains of Cell Surface N-Glycoproteins: Defining the Accessible Surfaceome for Immunophenotyping Stem Cells and Their Derivatives

      • Chelsea M. Fujinaka, Matthew Waas, Rebekah L. Gundry
      Pages 57-78

    6. Application of Higher Density Iron Oxide Nanoparticle Pellicles to Enrich the Plasma Membrane and Its Proteome from Cells in Suspension

      • Rebecca L. Rose, Waeowalee Choksawangkarn, Catherine Fenselau
      Pages 79-90

    7. Proteomic Profiling of Secreted Proteins, Exosomes, and Microvesicles in Cell Culture Conditioned Media

      • Ankit Sinha, Simona Principe, Javier Alfaro, Alex Ignatchenko, Vladimir Ignatchenko, Thomas Kislinger
      Pages 91-102

  3. Targeted Approaches for Surfaceome Content

    1. Front Matter

      Pages 103-103
      Download chapter PDF

    2. Cloning, Expression, and Purification of the Glycosylated Transmembrane Protein, Cation-Dependent Mannose 6-Phosphate Receptor, from Sf9 Cells Using the Baculovirus System

      • Linda J. Olson, Nancy M. Dahms
      Pages 105-116

    3. Bispecific Antibody Armed T Cells to Target Cancer Cells

      • Archana Thakur, Lawrence G. Lum, Sandeep Mittal
      Pages 117-126

    4. Immunophenotyping of Live Human Pluripotent Stem Cells by Flow Cytometry

      • Daniel R. Riordon, Kenneth R. Boheler
      Pages 127-149

    5. Detecting Cell Surface Expression of the G Protein-Coupled Receptor CXCR4

      • Amanda M. Nevins, Adriano Marchese
      Pages 151-164

  4. Cell-Based Functional Analyses Related to Surfaceome Content

    1. Front Matter

      Pages 165-165
      Download chapter PDF

    2. NaV Channels: Assaying Biosynthesis, Trafficking, Function

      • Gordon F. Tomaselli, Federica Farinelli
      Pages 167-184

    3. High-Content Electrophysiological Analysis of Human Pluripotent Stem Cell-Derived Cardiomyocytes (hPSC-CMs)

      • Chi-Wing Kong, Lin Geng, Ronald A. Li
      Pages 185-194

    4. Methods for Evaluation of Vascular Endothelial Cell Function with Transient Receptor Potential (TRP) Channel Drugs

      • Yung Wui Tjong, Xiaoqiang Yao
      Pages 195-210

    5. Methods to Study the Signal Transduction of the Surface Receptor Tyrosine Kinase TrkB in Neurons

      • Kwok-On Lai, Nancy Y. Ip
      Pages 211-222

    6. Polarized Human Retinal Pigment Epithelium Exhibits Distinct Surface Proteome on Apical and Basal Plasma Membranes

      • Vladimir Khristov, Qin Wan, Ruchi Sharma, Mostafa Lotfi, Arvydas Maminishkis, Kapil Bharti
      Pages 223-247

    7. Extracellular Matrix Molecule-Based Capture of Mesenchymal Stromal Cells Under Flow

      • Teresa Massam-Wu, Stuart A. Cain, Cay M. Kielty
      Pages 249-260
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Keywords

About this book

This volume provides readers with the latest techniques and tools to assess modifications and functions of the surfaceome. The chapters in this book are divided into 4 sections: discovery-based approaches to surfaceome content; targeted approaches for surfaceome content; cell-based function analyses related to surfaceome content; and computational approaches in surfaceome studies. Section 1 focuses on discovery-based approaches for cataloging surfaceome content that analyses the surfaceome of bacteria, avian embryos, and mammalian systems. Section 2 discusses methods that over-express specific targets in Sf9 cells and generate bi-specific antibodies for targeting cancer and somatic cells. Section 3 explores voltage dependent sodium channels and high-content electrophysiological analyses. The final section looks at the new web-based platform known as targets-search. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions totheir respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.



Comprehensive and thorough, Surfaceome: Methods and Protocols assists in the study of cell surface protein biology and function. It is a valuable resource for all researchers interested in this field.


Editors and Affiliations

  • School of Biomedical Sciences, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong, SAR, China

    Kenneth R. Boheler

  • Department of Biochemistry, Medical College of Wisconsin, Milwaukee, USA

    Rebekah L. Gundry

Bibliographic Information

  • Book Title: The Surfaceome

  • Book Subtitle: Methods and Protocols

  • Editors: Kenneth R. Boheler, Rebekah L. Gundry

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7553-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2018

  • Hardcover ISBN: 978-1-4939-7551-8Published: 20 December 2017

  • Softcover ISBN: 978-1-4939-8518-0Published: 30 August 2018

  • eBook ISBN: 978-1-4939-7553-2Published: 20 December 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 348

  • Number of Illustrations: 21 b/w illustrations, 47 illustrations in colour

  • Topics: Cell Biology

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