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The Surfaceome

Methods and Protocols

  • Kenneth R. Boheler
  • Rebekah L. Gundry

Part of the Methods in Molecular Biology book series (MIMB, volume 1722)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Discovery-Based Approaches to Surfaceome Content

    1. Front Matter
      Pages 1-1
    2. Yulanda M. Williamson, Jennifer Whitmon, Rolieria West-Deadwyler, Hercules Moura, Adrian R. Woolfitt, Jon Rees et al.
      Pages 3-20
    3. Ankit Sinha, Simona Principe, Javier Alfaro, Alex Ignatchenko, Vladimir Ignatchenko, Thomas Kislinger
      Pages 91-102
  3. Targeted Approaches for Surfaceome Content

  4. Cell-Based Functional Analyses Related to Surfaceome Content

    1. Front Matter
      Pages 165-165
    2. Gordon F. Tomaselli, Federica Farinelli
      Pages 167-184
    3. Vladimir Khristov, Qin Wan, Ruchi Sharma, Mostafa Lotfi, Arvydas Maminishkis, Kapil Bharti
      Pages 223-247
    4. Teresa Massam-Wu, Stuart A. Cain, Cay M. Kielty
      Pages 249-260
    5. Prasenjit Bose, Chen Yu Huang, Jeroen Eyckmans, Christopher S. Chen, Daniel H. Reich
      Pages 303-328
  5. Computational Approaches in Surfaceome Studies

  6. Back Matter
    Pages 345-347

About this book

Introduction

This volume provides readers with the latest techniques and tools to assess modifications and functions of the surfaceome. The chapters in this book are divided into 4 sections: discovery-based approaches to surfaceome content; targeted approaches for surfaceome content; cell-based function analyses related to surfaceome content; and computational approaches in surfaceome studies. Section 1 focuses on discovery-based approaches for cataloging surfaceome content that analyses the surfaceome of bacteria, avian embryos, and mammalian systems. Section 2 discusses methods that over-express specific targets in Sf9 cells and generate bi-specific antibodies for targeting cancer and somatic cells. Section 3 explores voltage dependent sodium channels and high-content electrophysiological analyses. The final section looks at the new web-based platform known as targets-search. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and thorough, Surfaceome: Methods and Protocols assists in the study of cell surface protein biology and function. It is a valuable resource for all researchers interested in this field.

Keywords

plasma membrane cell surface proteins antibodies lipids and glycans immunophenotyping markers avian embryos and mammalian systems Cell Surface Capture Technology n-glycoproteins G protein couple receptors CXCR4 fluid transport Targets-search bioinformatics

Editors and affiliations

  • Kenneth R. Boheler
    • 1
  • Rebekah L. Gundry
    • 2
  1. 1.School of Biomedical Sciences, LKS Faculty of MedicineThe University of Hong KongHong Kong, SARChina
  2. 2.Department of BiochemistryMedical College of WisconsinMilwaukeeUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-7553-2
  • Copyright Information Springer Science+Business Media, LLC 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7551-8
  • Online ISBN 978-1-4939-7553-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site