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Magnetic Resonance Spectroscopy Studies of Mouse Models of Cancer

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Preclinical MRI

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1718))

Abstract

Magnetic resonance spectroscopy (MRS) or spectroscopic imaging (MRSI) enables the detection of metabolites, amino acids, and lipids, among other biomolecules, in tumors of live mouse models of cancer. Tumor-bearing mice are anesthetized by breathing isoflurane in a magnetic resonance (MR) scanner dedicated to small animal MR. Here we describe the overall setup and steps for measuring 1H and 31P MRS and 1H MRSI of orthotopic breast tumor models in mice with surface coils. This protocol can be adapted to the use of volume coils to measure 1H and 31P MRS(I) of tumor models that grow inside the body. We address issues of animal handling, setting up the measurement, measurement options, and data analysis.

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Acknowledgements

This work was supported by NIH R01 CA134695, R01 CA154725, and P50 CA103175.

Author information

Authors and Affiliations

  1. Division of Cancer Imaging Research, Russell H. Morgan Department of Radiology and Radiological Science, Johns Hopkins University School of Medicine, Baltimore, MD, USA

    Menglin Cheng & Kristine Glunde

  2. Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD, USA

    Menglin Cheng & Kristine Glunde

Authors
  1. Menglin Cheng
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  2. Kristine Glunde
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Corresponding author

Correspondence to Kristine Glunde .

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Editors and Affiliations

  1. BIONAND, Andalusian Centre for Nanomedicine and Biotechnology, Junta de Andalucía, Universidad de Málaga, Málaga, Spain

    María Luisa García Martín

  2. Instituto de Investigaciones Biomédicas “Alberto Sols”, CSIC/UAM, Madrid, Spain

    Pilar López Larrubia

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Cheng, M., Glunde, K. (2018). Magnetic Resonance Spectroscopy Studies of Mouse Models of Cancer. In: García Martín, M., López Larrubia, P. (eds) Preclinical MRI. Methods in Molecular Biology, vol 1718. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7531-0_20

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  • DOI: https://doi.org/10.1007/978-1-4939-7531-0_20

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7530-3

  • Online ISBN: 978-1-4939-7531-0

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