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Quantification of Cell-Surface Glucose Transporters in the Heart Using a Biotinylated Photolabeling Assay

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Glucose Transport

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1713))

Abstract

The biotinylated photolabeling assay enables quantification of cell-surface glucose transporters (GLUTs). This technique has been successfully applied to quantify the cell-surface GLUT protein content in striated muscles and adipose tissue, as a means to evaluate GLUT trafficking. Here, we describe the detailed method of quantifying the cell-surface content of several GLUT isoforms (1, 4, 8, and 12) in isolated cardiac myocytes, as well as in the intact perfused atria and ventricle.

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Acknowledgements

We thank Allison Campolo, MS, for carefully reading the manuscript. This research was funded by National Institute of Health (K01RR023083), the Harold Hamm Diabetes Research Center, The Oklahoma State University Center of Veterinary Health Sciences, and Oklahoma Center of Respiratory and Infectious Disease (NIH 1P20 GM103648).

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Authors and Affiliations

  1. Department of Physiological Sciences, Oklahoma State University, 283 McElroy Hall, Stillwater, OK, 74078, USA

    Zahra Maria & Véronique A. Lacombe

  2. Harold Hamm Diabetes Center, University of Oklahoma Health Science Center, Oklahoma City, OK, USA

    Zahra Maria & Véronique A. Lacombe

Authors
  1. Zahra Maria
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  2. Véronique A. Lacombe
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Corresponding author

Correspondence to Véronique A. Lacombe .

Editor information

Editors and Affiliations

  1. Department of Experimental Medical Science, Lund University, Lund, Sweden

    Karin Lindkvist-Petersson

  2. Department of Experimental Medical Science, Lund University, Lund, Sweden

    Jesper S Hansen

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Maria, Z., Lacombe, V.A. (2018). Quantification of Cell-Surface Glucose Transporters in the Heart Using a Biotinylated Photolabeling Assay. In: Lindkvist-Petersson, K., Hansen, J. (eds) Glucose Transport. Methods in Molecular Biology, vol 1713. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7507-5_17

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  • DOI: https://doi.org/10.1007/978-1-4939-7507-5_17

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7506-8

  • Online ISBN: 978-1-4939-7507-5

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