Advertisement

Glucose Transport

Methods and Protocols

  • Karin Lindkvist-Petersson
  • Jesper S  Hansen

Part of the Methods in Molecular Biology book series (MIMB, volume 1713)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Raminta Venskutonytė, Karin Elbing, Karin Lindkvist-Petersson
    Pages 1-13
  3. Wentong Long, Debbie O’Neill, Chris I. Cheeseman
    Pages 45-55
  4. Eunice E. Lee, Richard C. Wang
    Pages 57-67
  5. Changyong Wei, Monique Heitmeier, Paul W. Hruz, Mala Shanmugam
    Pages 69-75
  6. Jesper S. Hansen, Karin Lindkvist-Petersson
    Pages 77-91
  7. Carlotta Granchi, Tiziano Tuccinardi, Filippo Minutolo
    Pages 93-108
  8. Niek Welkenhuysen, Caroline B. Adiels, Mattias Goksör, Stefan Hohmann
    Pages 109-121
  9. Eckhard Boles, Mislav Oreb
    Pages 123-135
  10. Sebastian Wasserstrom, Björn Morén, Karin G. Stenkula
    Pages 151-159
  11. Robert M. Jackson, Ann Louise Olson
    Pages 161-173
  12. Javier R. Jaldin-Fincati, Philip J. Bilan, Amira Klip
    Pages 175-192
  13. Dougall M. Norris, Tom A. Geddes, David E. James, Daniel J. Fazakerley, James G. Burchfield
    Pages 193-215
  14. Dimitrios Kioumourtzoglou, Gwyn W. Gould, Nia J. Bryant
    Pages 217-227
  15. Sabina Tsytkin-Kirschenzweig, Merav Cohen, Yaakov Nahmias
    Pages 241-254
  16. Gwenaël Labouèbe, Bernard Thorens, Christophe Lamy
    Pages 255-267
  17. Back Matter
    Pages 269-274

About this book

Introduction

This volume explores methods used to examine the structure-function relationship of glucose transporters both in vitro and ex vivo. Chapters in this book cover topics such as expression and purification of glucose transporters in heterologous expression systems, crystal structure determination, glucose transport activity assays in cellular and synthetic model systems, investigation of the GLUT4 membrane trafficking machinery by novel labeling strategies combined with advanced fluorescence microscopy methods, and tracking GLUT2 translocation by live cell imaging. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Glucose Transport: Methods and Protocols is a valuable resource that helps researchers with determining specific roles of different GLUTs in various organisms.

Keywords

GLUTs SGLTs yeast FRET-Based Assay hexose transport proteins

Editors and affiliations

  • Karin Lindkvist-Petersson
    • 1
  • Jesper S  Hansen
    • 2
  1. 1.Department of Experimental Medical ScienceLund UniversityLundSweden
  2. 2.Department of Experimental Medical ScienceLund UniversityLundSweden

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-7507-5
  • Copyright Information Springer Science+Business Media LLC 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7506-8
  • Online ISBN 978-1-4939-7507-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site