Abstract
Chromatin immunoprecipitation (ChIP) is considered the method of choice for characterizing interactions between a protein of interest and specific genomic regions. It is of paramount importance in gene-regulation studies, as it can be used to map the target regions of sequence-specific transcription factors and cofactors, or histone marks that characterize distinct chromatin states. ChIP can be used directly to probe interactions with candidate regions (ChIP-PCR), or coupled to Next-Generation Sequencing (ChIP-seq) to generate genome-wide information. This chapter describes a protocol for performing ChIP and ChIP-seq of transcription factors, starting either from mouse embryonic tissue or adherent cells in culture.
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Soares, M.A.F., Castro, D.S. (2018). Chromatin Immunoprecipitation from Mouse Embryonic Tissue or Adherent Cells in Culture, Followed by Next-Generation Sequencing. In: Visa, N., Jordán-Pla, A. (eds) Chromatin Immunoprecipitation. Methods in Molecular Biology, vol 1689. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7380-4_5
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DOI: https://doi.org/10.1007/978-1-4939-7380-4_5
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7379-8
Online ISBN: 978-1-4939-7380-4
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