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Overview of Methods for Assessing Salinity and Drought Tolerance of Transgenic Wheat Lines

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Wheat Biotechnology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1679))

Abstract

Salinity and drought are interconnected, causing phenotypic, physiological, biochemical, and molecular changes in a cell. These stresses are the major factors adversely affecting growth and productivity in cereals. Genetic engineering methods have advanced to enable development of genotypes with improved salinity and drought tolerance. The resulting transgenic plant produces a group of progenies which includes moderate to high-stress tolerant transgenic lines. Development of reproducible screening methods to identify high-stress tolerant germplasm under laboratory, greenhouse, or field conditions is must. Further, field level demonstration of improved phenotypes and yield under salinity and drought stress conditions is both challenging and expensive. Fast and efficient screening techniques that could be used to screen transgenic lines under greenhouse conditions, for salt and drought stress tolerance, may contribute toward the identification of promising lines for field conditions. This chapter provides information on various approaches which can be developed during different stages of plant development for selecting salinity and drought tolerant plants in cereals, especially wheat.

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Acknowledgments

R.J. acknowledges Dr. D.S. Kothari Postdoctoral Fellowship from University Grant Commission, Government of India. A.P. would like to acknowledge the financial support received from UGC-RNW and UOE-II from Jawaharlal Nehru University, JNU (India). Research in the lab of A.P. also supported from funds received from International Atomic Energy Agency (Vienna), India-NWO, DBT and Indo-US Science and Technology Forum (IUSSTF), New, Delhi.

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Authors and Affiliations

  1. Stress Physiology and Molecular Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi, 110067, Delhi, India

    Rohit Joshi, Khalid Anwar, Priyanka Das & Ashwani Pareek

  2. Plant Stress Biology Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110067, Delhi, India

    Sneh L. Singla-Pareek

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  1. Rohit Joshi
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  2. Khalid Anwar
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  3. Priyanka Das
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  4. Sneh L. Singla-Pareek
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  5. Ashwani Pareek
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Correspondence to Ashwani Pareek .

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Editors and Affiliations

  1. Plant Molecular Biology and Biotechnology Laboratory, School of Agriculture and Food, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria, Australia

    Prem L. Bhalla

  2. Plant Molecular Biology and Biotechnology Laboratory, School of Agriculture and Food, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria, Australia

    Mohan B. Singh

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Joshi, R., Anwar, K., Das, P., Singla-Pareek, S.L., Pareek, A. (2017). Overview of Methods for Assessing Salinity and Drought Tolerance of Transgenic Wheat Lines. In: Bhalla, P., Singh, M. (eds) Wheat Biotechnology. Methods in Molecular Biology, vol 1679. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7337-8_5

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  • DOI: https://doi.org/10.1007/978-1-4939-7337-8_5

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