Abstract
The male germline of flowering plants develops within the vegetative cell of the male gametophyte and displays a distinct transcriptional profile. Key to understanding the development of this unique cell lineage is determining how gene expression is regulated within germline cells. This knowledge impacts upon our understanding of cell specification, differentiation, and plant fertility. Here, we describe methods to identify cis-regulatory modules (CRMs) that act as key regulatory regions in the promoters of germline-expressed genes. We detail the complimentary techniques of phylogenetic footprinting and the use of fluorescent reporters in pollen for the identification and verification of CRMs.
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Acknowledgments
This work was supported by a Fast Start grant from the Marsden Fund of the Royal Society of New Zealand (12-UOO-043) to L.B. and by research grants from the Biotechnology and Biological Sciences Research Council (BB/I011269/1; BB/N005090/1) to D.T. B.P. was supported by a University of Otago Doctoral Scholarship.
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Peters, B., Aidley, J., Cadzow, M., Twell, D., Brownfield, L. (2017). Identification of Cis-Regulatory Modules that Function in the Male Germline of Flowering Plants. In: Schmidt, A. (eds) Plant Germline Development. Methods in Molecular Biology, vol 1669. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7286-9_22
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DOI: https://doi.org/10.1007/978-1-4939-7286-9_22
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