Abstract
We present high-throughput single-molecule manipulation using a benchtop centrifuge, overcoming limitations common in other single-molecule approaches such as high cost, low throughput, technical difficulty, and strict infrastructure requirements. An inexpensive and compact Centrifuge Force Microscope (CFM) adapted to a commercial centrifuge enables use by nonspecialists, and integration with DNA nanoswitches facilitates both reliable measurements and repeated molecular interrogation. Here, we provide detailed protocols for constructing the CFM, creating DNA nanoswitch samples, and carrying out single-molecule force measurements.
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Acknowledgement
W.P.W. acknowledges support from the Arnold and Mabel Beckman Foundation through the Beckman Young Investigator Award, US National Institutes of Health NIGMS R21 RGM107907A and R35 GM119537, the Wyss Institute at Harvard University and Boston Children’s Hospital startup funds.
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Yang, D., Wong, W.P. (2018). Repurposing a Benchtop Centrifuge for High-Throughput Single-Molecule Force Spectroscopy. In: Peterman, E. (eds) Single Molecule Analysis. Methods in Molecular Biology, vol 1665. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7271-5_19
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DOI: https://doi.org/10.1007/978-1-4939-7271-5_19
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