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Photorespiration

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Alisdair R. Fernie

    1. Max Planck Institute of Molecular Plant Physiology, Potsdam-Golm, Germany

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    You can also search for this editor in PubMed   Google Scholar

  2. Hermann Bauwe

    1. Plant Physiology Department, University of Rostock, Rostock, Germany

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    You can also search for this editor in PubMed   Google Scholar

  3. Andreas P.M. Weber

    1. Institute of Plant Biochemistry, Cluster of Excellence on Plant Sciences (CEPLAS), Heinrich-Heine-University, Düsseldorf, Germany

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1653)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Estimation of Photorespiratory Fluxes by Gas Exchange

    • Florian A. Busch, Ross M. Deans, Meisha-Marika Holloway-Phillips
    Pages 1-15

  3. Measurement of Transcripts Associated with Photorespiration and Related Redox Signaling

    • Amna Mhamdi, Pavel I. Kerchev, Patrick Willems, Graham Noctor, Frank Van Breusegem
    Pages 17-29

  4. Measurement of Enzyme Activities

    • Hermann Bauwe
    Pages 31-50

  5. In Vitro Alkylation Methods for Assessing the Protein Redox State

    • Flavien Zannini, Jérémy Couturier, Olivier Keech, Nicolas Rouhier
    Pages 51-64

  6. Dimethyl-Labeling-Based Quantification of the Lysine Acetylome and Proteome of Plants

    • Ines Lassowskat, Markus Hartl, Fabian Hosp, Paul J. Boersema, Matthias Mann, Iris Finkemeier
    Pages 65-81

  7. In Vitro Analysis of Metabolite Transport Proteins

    • Marc-Sven Roell, Franziska Kuhnert, Shirin Zamani-Nour, Andreas P. M. Weber
    Pages 83-96

  8. Quantification of Photorespiratory Intermediates by Mass Spectrometry-Based Approaches

    • Stéphanie Arrivault, Toshihiro Obata
    Pages 97-104

  9. Targeted Isolation and Characterization of T-DNA Mutants Defective in Photorespiration

    • Stefan Timm, Katharina Modde, Hermann Bauwe
    Pages 105-124

  10. Exploiting Natural Variation to Discover Candidate Genes Involved in Photosynthesis-Related Traits

    • Franklin Magnum de Oliveira Silva, Lucas de Ávila Silva, Wagner L. Araújo, Agustin Zsögön, Adriano Nunes-Nesi
    Pages 125-135

  11. Metabolic Engineering of Photorespiration

    • Martin K. M. Engqvist, Veronica G. Maurino
    Pages 137-155

  12. 13CO2 Labeling and Mass Spectral Analysis of Photorespiration

    • Leonardo Perez de Souza, Marek Szecówka, Alisdair R. Fernie, Takayuki Tohge
    Pages 157-166

  13. Isotopically Nonstationary Metabolic Flux Analysis (INST-MFA) of Photosynthesis and Photorespiration in Plants

    • Fangfang Ma, Lara J. Jazmin, Jamey D. Young, Doug K. Allen
    Pages 167-194

  14. Genome-Scale Modeling of Photorespiratory Pathway Manipulation

    • Anika Küken, Zoran Nikoloski
    Pages 195-202

  15. Kinetic Modeling of Photorespiration

    • Honglong Zhao, Yi Xiao, Xin-Guang Zhu
    Pages 203-216

  16. Investigating the Role of the Photorespiratory Pathway in Non-photosynthetic Tissues

    • Alisdair R. Fernie, Hermann Bauwe, Lee J. Sweetlove
    Pages 217-225

  17. Studying the Function of the Phosphorylated Pathway of Serine Biosynthesis in Arabidopsis thaliana

    • Stephan Krueger, Ruben M. Benstein, Sabine Wulfert, Armand D. Anoman, María Flores-Tornero, Roc Ros
    Pages 227-242

  18. Light Microscopy, Transmission Electron Microscopy, and Immunohistochemistry Protocols for Studying Photorespiration

    • Roxana Khoshravesh, Vanessa Lundsgaard-Nielsen, Stefanie Sultmanis, Tammy L. Sage
    Pages 243-270

  19. Back Matter

    Pages 271-274
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Keywords

About this book

This volume presents a comprehensive overview of contemporary methods to analyze photorespiration in higher plants. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Photorespiration: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • Max Planck Institute of Molecular Plant Physiology, Potsdam-Golm, Germany

    Alisdair R. Fernie

  • Plant Physiology Department, University of Rostock, Rostock, Germany

    Hermann Bauwe

  • Institute of Plant Biochemistry, Cluster of Excellence on Plant Sciences (CEPLAS), Heinrich-Heine-University, Düsseldorf, Germany

    Andreas P.M. Weber

Bibliographic Information

  • Book Title: Photorespiration

  • Book Subtitle: Methods and Protocols

  • Editors: Alisdair R. Fernie, Hermann Bauwe, Andreas P.M. Weber

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7225-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-7224-1Published: 19 August 2017

  • Softcover ISBN: 978-1-4939-8422-0Published: 03 August 2018

  • eBook ISBN: 978-1-4939-7225-8Published: 17 August 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 274

  • Number of Illustrations: 15 b/w illustrations, 25 illustrations in colour

  • Topics: Plant Sciences

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