Abstract
mRNA translation is a key step in decoding the genetic information stored in DNA. Regulation of translation efficiency contributes to gene expression control and is therefore important for cell fate and function. Here, we describe a recently developed microscopy-based method that allows for visualization of translation of single mRNAs in live cells. The ability to measure translation dynamics of single mRNAs will enable a better understanding of spatiotemporal control of translation, and will provide unique insights into translational heterogeneity of different mRNA molecules in single cells.
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Acknowledgment
This work was supported by a by a fellowship of the Dutch Cancer Society (KWF) to M.E.T. and a Veni grant from the Netherlands Organization for Scientific Research (NWO) to S.R.
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Ruijtenberg, S., Hoek, T.A., Yan, X., Tanenbaum, M.E. (2018). Imaging Translation Dynamics of Single mRNA Molecules in Live Cells. In: Gaspar, I. (eds) RNA Detection. Methods in Molecular Biology, vol 1649. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7213-5_26
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DOI: https://doi.org/10.1007/978-1-4939-7213-5_26
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7212-8
Online ISBN: 978-1-4939-7213-5
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