RNA Detection

Methods and Protocols

  • Imre Gaspar

Part of the Methods in Molecular Biology book series (MIMB, volume 1649)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Caroline Medioni, Florence Besse
    Pages 1-28
  3. Toshiaki Shigeoka, Jane Jung, Christine E. Holt, Hosung Jung
    Pages 85-94
  4. Susanne Nichterwitz, Julio Aguila Benitez, Rein Hoogstraaten, Qiaolin Deng, Eva Hedlund
    Pages 95-110
  5. Kaia Achim, Hernando Martínez Vergara, Jean-Baptiste Pettit
    Pages 111-125
  6. Shawn C. Little, Thomas Gregor
    Pages 127-142
  7. L. Carine Stapel, Coleman Broaddus, Nadine L. Vastenhouw
    Pages 143-162
  8. Joshua S. Titlow, Lu Yang, Richard M. Parton, Ana Palanca, Ilan Davis
    Pages 163-175 Open Access
  9. Tomasz Krzywkowski, Mats Nilsson
    Pages 209-229
  10. Yantao Yang, Mingming Chen, Christopher J. Krueger, Andrew Tsourkas, Antony K. Chen
    Pages 231-242
  11. Mingming Chen, Yantao Yang, Christopher J. Krueger, Antony K. Chen
    Pages 243-257
  12. Jasmine Chamiolo, Imre Gaspar, Anne Ephrussi, Oliver Seitz
    Pages 273-287
  13. Murat Sunbul, Ankita Arora, Andres Jäschke
    Pages 289-304

About this book

Introduction

This volume introduces different concepts and methods of detecting RNA in biological material in a variety of model systems. The chapters in this book discuss methods that will answer numerous biological questions that arise in the study of RNAs. Some of the topics covered in this book are single mRNA molecule detection in embryos and neurons; detection of mRNA and associated molecules by ISH-IEM on frozen sections; optimizing molecular beacons for intracellular analysis of RNA; imaging translation dynamics of single mRNA molecules in live cells; preparation of high-throughput sequencing libraries; and capturing RNA binding proteins in embryos and in cell-culture. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, RNA Detection: Methods and Protocols is a valuable resource for novel and experiences scientist in the expanding field of RNAs.

Keywords

Drosophila ECHO-liveFISH FISH RCas9 FISSEQ

Editors and affiliations

  • Imre Gaspar
    • 1
  1. 1.Developmental Biology UnitEMBL HeidelbergHeidelbergGermany

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-7213-5
  • Copyright Information Springer Science+Business Media LLC 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7212-8
  • Online ISBN 978-1-4939-7213-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book