Abstract
Date palm fruit production has great economic significance for many countries. There is a fundamental necessity to conserve valuable date palm germplasm, but there are various problems with in vivo and ex situ conservation. In vitro storage has several advantages over conventional germplasm conservation methods. The in vitro technique offers a developed method of slow-growth storage, which is considered as an alternate solution for short- and medium-term storage of date palm germplasm under controlled conditions. Minimal growth conditions for germplasm conservation are generally achieved by reducing growth rate through modification of environmental growing conditions and culture, by using low temperatures, and the addition of growth retardants and osmotic agents. This chapter describes a protocol for short-term in vitro conservation of date palm shoot-tip and callus cultures under slow-growth storage conditions, using sucrose as an osmotic agent and abscisic acid (ABA) as a growth retardant at 15 °C for 12 months.
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References
Al-Khayri JM (2007) Date palm Phoenix dactylifera L. micropropagation. In: Jain SM, Haggman H (eds) Protocols for micropropagation of woody trees and fruits. Springer, Berlin, pp 509–526
El-Dawayati MM (2000) Physiological studies on date palm micropropagation. M.Sc. Thesis, Department of Pomology, Faculty of Agriculture, Cairo University, Egypt
Al-Khayri JM (2010) Somatic embryogenesis of date palm Phoenix dactylifera L. improved by coconut water. Biotechnolgy 9:477–484
Abahmane L (2011) Date palm micropropagation via organogenesis. In: Jain SM, Al-Khayri JM, Johnson DV (eds) Date palm biotechnology. Springer, Dordrecht, pp 69–90
Zayed Z (2014) Effect of different types of cytokinins on the regeneration ability of direct somatic embryos and adventitious shoots induced immature inflorescences of date palm. Egy J Appl Sci 29(3):142–153
Jazinizadeh E, Zarghami R, Majd A, Iranbakhsh A, Tajaddod A (2015) In vitro production of date palm Phoenix dactylifera L. cv. ‘Barhee’ plantlets through direct organogenesis. Biol Forum 7(2):566–572
Bekheet SA, Taha HS (2013) Complementary strategy for conservation of date palm germplasm. Glob J Biodivers Sci Manag 3(1):96–107
Gianní S, Sottile F (2015) In vitro storage of plum germplasm by slow growth. Hort Sci 42(2):61–69
Scherwinski Pereira JE, Costa FH, Camillo J, Silva DB, Alves RB, Vieira RF (2010) Tissue culture storage of Brazilian medicinal plants germplasm. Acta Hort (860):211–214
Bekheet SA, Taha HS, Saker MM (2001) In vitro long-term storage of date palm. Biol Plant 45:121–124
Hassan M (2002) In vitro studies on somatic embryogenesis conservation of date palm. Ph.D. Thesis Department of Pomology, Faculty of Agriculture, Cairo University, Egypt
El-Dawayati MM (2008) Using tissue culture technology to storage some plant tissues of date palm. Ph.D. Thesis, Department of Pomology, Faculty of Agriculture, Cairo University, Egypt
Engelmann F (2011) Use of biotechnologies for the conservation of plant biodiversity. In Vitro Cell Dev Biol Plant 45:5–16
El-Dawayati MM, Baker EI, Gomaa AH, Zayed Z (2013) In vitro conservation of date palm shoot tip explants under minimal growth condition. Egypt J Agric Res 91(3):1043–1062
El-Ashry AA, Shaltout AD, El-Bahr MK, Abd El-Hamid A, Bekheet SA (2013) In vitro preservation of embryogenic callus cultures of two Egyptian dry date palm cultivars at darkness and low temperature conditions. J Hort Sci Ornam Plants 5(2):118–126
George EF, Hall MA, Klerk GJ (2008) The components of plant tissue culture media 1: macro and micro-nutrients. In: George EF, Hall MA, Klerk GJ (eds) Plant propagation by tissue culture. Springer, Dordrecht, pp 65–113
El-Dawayati MM, Zayed Z, Baker EI, Gomaa AH (2011) Studies on storage under minimal growth conditions of date palm callus explants. In: Proceedings first scientific conference for the development of the date palm and dates sector in the Arab world. King Abdulaziz, City for Science and Technology, Kingdom of Saudi Arabia, 4–7 Dec 2011, pp 401–420
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Acknowledgment
The author wishes to acknowledge the researcher Mr. Mahmoud Hamza for the vital help to achieve this work.
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El-Dawayati, M.M. (2017). In Vitro Conservation of Date Palm Shoot-Tip Explants and Callus Cultures Under Minimal Growth Conditions. In: Al-Khayri, J., Jain, S., Johnson, D. (eds) Date Palm Biotechnology Protocols Volume II. Methods in Molecular Biology, vol 1638. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7159-6_5
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DOI: https://doi.org/10.1007/978-1-4939-7159-6_5
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7158-9
Online ISBN: 978-1-4939-7159-6
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