Abstract
In vitro technology offers a potential solution for the conservation of date palm germplasm. Slow growth induced by low temperature allows storage from several months up to few years. Otherwise, cryopreservation is suitable for long-term in vitro conservation, at between −79 and −196 °C. This chapter describes a protocol for cold storage at 5 °C and cryopreservation of date palm tissue cultures. For cold storage, 70% of shoot buds remain healthy after storing for 12 months at 5 °C, and callus cultures remain fully viable after 12 months of storage. For cryopreservation of embryogenic cultures using dehydration by air, apparently, 20 min air drying is the best for cryopreservation. Among different types of sugars used as osmotic agents in pre-culture medium, 1 M sucrose is the best for the survival of cryopreserved cultures. However, exposure of embryogenic cultures to vitrification solution for 60 min at 0 °C gives the highest percentage of survival and conversion to plantlets.
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References
Tandon P, Kumaria S (2005) Prospects of plant conservation biotechnology in India with special reference to northeastern region. In: Tandon P, Kumaria S (eds) Biodiversity: status and prospects. Norasa Publishing House, New Delhi, pp 79–91
Silva R de C, Gomes LZ, Schrewinski-Pereira JE (2012) Short-term storage in vitro and large-scale propagation of grapevine genotypes. Pesq Agropec Bras 47(3):344–350
Bekheet SA, Ibrahim UA (2007) In vitro conservation of globe artichoke (Cynara scolymus L.) Int J Agric Biol 9(3):404–407
Hassan NA, Bekheet SA (2008) Mid-term storage and genetic stability of strawberry tissue cultures. Res J Agric Biol Sci 4(5):505–511
Sharaf SA, Shibli AR, Kasrawi AM, Baghdadi SH (2012) Slow-growth preservation of wild shih (Artemisia herba-alba Asso.) microshoots from Jordan. J Food Agri Environ 10(2):1359–1364
Shibli RA, Shatnawi MA, Ajlouni MM, Jaradat A, Adham Y (1999) Slow growth in vitro conservation of the bitter almond (Amygdalus communis L.) Adv Hortic Sci 13:133–134
Fortes GR, Pereira JES (2001) Effect of low temperature and growth retardants on in vitro conservation of asparagus. Revista Cient Rural 6:181–186
Engelmann F (2011) Use of biotechnologies for the conservation of plant biodiversity. In Vitro Cell Dev Biol Plant 47:5–16
Mandumpal JB, Kreck CA, Mancera RL (2011) A molecular mechanism of solvent cryoprotection in aqueous DMSO solutions. Phys Chem Chem Phys 13:3839–3842
Panis B, Strosse H, Van den Hende S, Swennen R (2002) Sucrose preculture to simplify cryopreservation of banana meristem cultures. Cryo-Lett 23:375–384
Moges AD, Karam NS, Shibli RA (2004) Cryopreservation of African violet (Saintpaulia ionantha Wendl.) shoot tips. In Vitro Cell Dev Biol Plant 40:389–398
Wedley-Smith J, Berjak P, Pammenter NW, Walters C (2014) Intracellular ice and cell survival in cryo-exposed embryonic aves of Acer saccharinum: an ultrastructural study of factors affecting cell and ice structures. Ann Bot 113:695–709
Bekheet SA, Taha HS, Saker MM (2001) In vitro long-term storage of date palm. Biol Plant 45:121–124
El-Ashry AA, Shaltout AD, El-Bahr MK, Abd EL-Hamid A, Matter MA, Bekheet SA (2013) In vitro preservation of embryogenic callus cultures of two Egyptian dry date palm cultivars at darkness and low temperature conditions. J Appl Sci Res 9(3):1985–1992
El-Bahr MK, Abd El-Hamid A, Matter MA, Shaltout A, Bekheet SA, El-Ashry AA (2016) In vitro conservation of embryogenic cultures of date palm using osmotic mediated growth agents. J Genet Engin Biotech 14(2):363–370
Bekheet SA, Taha HS, Solliman ME, Hassan NA (2007) Cryopreservation of date palm (Phoenix dactylifera L.) cultured in vitro. Acta Hortic 736:283–291
Bekheet SA (2015) Effect of cryopreservation on salt and drought tolerance of date palm cultured in vitro. Sci Agric 9(3):142–149
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
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Bekheet, S.A. (2017). In Vitro Conservation of Date Palm Tissue Cultures. In: Al-Khayri, J., Jain, S., Johnson, D. (eds) Date Palm Biotechnology Protocols Volume II. Methods in Molecular Biology, vol 1638. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7159-6_2
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DOI: https://doi.org/10.1007/978-1-4939-7159-6_2
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Online ISBN: 978-1-4939-7159-6
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