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Native Mass Spectrometry for the Characterization of Structure and Interactions of Membrane Proteins

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Membrane Protein Structure and Function Characterization

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1635))

Abstract

Over the past years, native mass spectrometry and ion mobility have grown into techniques that are widely applicable to the study of aspects of protein structure. More recently, it has become apparent that this approach provides a very promising avenue for the investigation of integral membrane proteins in lipid or detergent environments.

In this chapter, we discuss applications of native mass spectrometry and ion mobility in membrane protein research—what is important to take into consideration when working with membrane proteins, and what the requirements are for sample preparation for native mass spectrometry. Furthermore, we will discuss the types of information provided by the measurements, including the oligomeric state, subunit composition and stoichiometry, interactions with detergents or lipids, conformational transitions, and the binding and structural effect of ligands and drugs.

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Authors and Affiliations

  1. Biomolecular and Analytical Mass Spectrometry Group, Chemistry Department, University of Antwerp, Groenenborgerlaan 171, 2020, Antwerp, Belgium

    Jeroen F. van Dyck, Albert Konijnenberg & Frank Sobott

  2. Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds, UK

    Frank Sobott

  3. School of Molecular and Cellular Biology, University of Leeds, Leeds, UK

    Frank Sobott

Authors
  1. Jeroen F. van Dyck
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  2. Albert Konijnenberg
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  3. Frank Sobott
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Correspondence to Frank Sobott .

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Editors and Affiliations

  1. Département de Chimie, CNRS UMR 7203 LBM, Sorbonne Universités—UPMC Univ Paris 06, École Normale Supérieure—PSL Research University, Paris, France

    Jean-Jacques Lacapere

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van Dyck, J.F., Konijnenberg, A., Sobott, F. (2017). Native Mass Spectrometry for the Characterization of Structure and Interactions of Membrane Proteins. In: Lacapere, JJ. (eds) Membrane Protein Structure and Function Characterization. Methods in Molecular Biology, vol 1635. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7151-0_11

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  • DOI: https://doi.org/10.1007/978-1-4939-7151-0_11

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