Abstract
Extracellular vesicles (EVs) play a key role in cell-to-cell communication carrying lipids, genetic material, and proteins, producing changes in their target cells. In the last years, EVs have been a hot topic owning to their potential source of biomarkers in many diseases. The search for new biomarkers of central nervous diseases is limited due to the difficulty in accessing to biopsies or complications after obtaining cerebrospinal fluid. In this sense, the study of EVs obtained from blood is proposed as a source of surrogate biomarkers of easy access. One of the most studied components of EVs as biomarkers is their protein cargo. In this chapter we present the main protocols for EV isolation from blood and urine samples, these being based on differential centrifugation and commercial kits for polymeric precipitation. Subsequently, the protocol for a proteomic characterization of plasma-derived EVs is also detailed.
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Sáenz-Cuesta, M., Santamaría, E., Fernández-Irigoyen, J., Otaegui, D. (2017). Proteomic Analysis of Extracellular Vesicles in Neurological Diseases. In: Santamaría, E., Fernández-Irigoyen, J. (eds) Current Proteomic Approaches Applied to Brain Function. Neuromethods, vol 127. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7119-0_15
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DOI: https://doi.org/10.1007/978-1-4939-7119-0_15
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Online ISBN: 978-1-4939-7119-0
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