Book Volume 127 2017

Current Proteomic Approaches Applied to Brain Function

Editors:

ISBN: 978-1-4939-7118-3 (Print) 978-1-4939-7119-0 (Online)

Table of contents (20 protocols)

  1. Front Matter

    Pages i-xvi

  2. Introduction

    1. Front Matter

      Pages 1-1

    2. No Access

      Protocol

      Pages 3-7

      Brain Proteomics: Decoding Neuroproteomes Using Mass Spectrometry

  3. Labeling Methods in Neuroproteomics

    1. Front Matter

      Pages 9-9

    2. No Access

      Protocol

      Pages 11-28

      Applications of Amine-Reactive Tandem Mass Tags (TMT) in Human Neuroproteomics

    3. No Access

      Protocol

      Pages 29-42

      Application of Isobaric Tags for Relative and Absolute Quantitation (iTRAQ) to Monitor Olfactory Proteomes During Alzheimer’s Disease Progression

    4. No Access

      Protocol

      Pages 43-62

      Protein Microarrays in Neurodegenerative Diseases

  4. Label-Free Methods in Neuroproteomics

    1. Front Matter

      Pages 63-63

    2. No Access

      Protocol

      Pages 65-74

      Comprehensive Shotgun Proteomic Analyses of Oligodendrocytes Using Ion Mobility and Data-Independent Acquisition

    3. No Access

      Protocol

      Pages 75-90

      Nontargeted Brain Lipidomic Profiling Performed by UPLC-ESI-qToF-MS/MS

    4. No Access

      Protocol

      Pages 91-106

      Methods for Human Olfactory Bulb Tissue Studies Using Peptide/Protein MALDI-TOF Imaging Mass Spectrometry (MALDI-IMS)

    5. No Access

      Protocol

      Pages 107-138

      Neuroproteomics Using Short GeLC-SWATH: From the Evaluation of Proteome Changes to the Clarification of Protein Function

  5. Mass Spectrometry-Based Neuroproteomics to Analyze Post-Translational Modificiations

    1. Front Matter

      Pages 139-139

    2. No Access

      Protocol

      Pages 141-159

      Analysis of Brain Phosphoproteome Using Titanium Dioxide Enrichment and High-Resolution LC-MS/MS

    3. No Access

      Protocol

      Pages 161-177

      N-Glycomics and N-Glycoproteomics of Human Cerebrospinal Fluid

    4. No Access

      Protocol

      Pages 179-189

      In Vivo Strategies to Isolate and Characterize the Neuronal Ubiquitinated Proteome

    5. No Access

      Protocol

      Pages 191-206

      Characterization of the Phosphoproteome and Sialoproteome in Brain Tissues by Mass Spectrometry

    6. No Access

      Protocol

      Pages 207-224

      Proteomic Analysis of SUMOylation in the Post-ischemic Brain

    7. No Access

      Protocol

      Pages 225-241

      S-Nitrosylation in Alzheimer’s Disease Using Oxidized Cysteine-Selective cPILOT

  6. Subcellular Neuroproteomics

    1. Front Matter

      Pages 243-243

    2. No Access

      Protocol

      Pages 245-253

      Proteomic Analysis of Extracellular Vesicles in Neurological Diseases

    3. No Access

      Protocol

      Pages 255-280

      Quantitative In-Depth Profiling of the Postsynaptic Density Proteome to Understand the Molecular Mechanisms Governing Synaptic Physiology and Pathology

    4. No Access

      Protocol

      Pages 281-288

      Nuclear Proteomics for Exploring MK-801-Treated Oligodendrocytes to Better Understand Schizophrenia

    5. No Access

      Protocol

      Pages 289-301

      Localized Proteomics of Individual Neurons Isolated from Formalin-Fixed, Paraffin-Embedded Tissue Sections Using Laser Capture Microdissection

  7. Bioinformatics

    1. Front Matter

      Pages 303-303

    2. No Access

      Protocol

      Pages 305-324

      Creation of Reusable Bioinformatics Workflows for Reproducible Analysis of LC-MS Proteomics Data

    3. No Access

      Protocol

      Pages 325-356

      Integration of Transcriptomic and Proteomic Data for Disease Insights

  8. Back Matter

    Pages 357-360