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Rapid Antibiotic Susceptibility Testing for Urinary Tract Infections

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Diagnostic Bacteriology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1616))

Abstract

Antibiotic susceptibility testing is important to guide clinicians in their choice of antibiotic used for treatment of bacterial infections. Current methods are time-consuming and more rapid alternatives are needed. Here, we describe a novel rapid method for antibiotic susceptibility testing which combines phenotypic and genotypic measurements. The use of padlock probes and rolling circle amplification allows for fast and precise determination of antibiotic susceptibilities as well as species identification.

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References

  1. Costelloe C, Metcalfe C, Lovering A, Mant D, Hay AD (2010) Effect of antibiotic prescribing in primary care on antimicrobial resistance in individual patients: systematic review and meta-analysis. BMJ 340:c2096. doi:10.1136/bmj.c2096. bmj.c2096 [pii]

    Article  PubMed  Google Scholar 

  2. Wiegand I, Hilpert K, Hancock RE (2008) Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances. Nat Protoc 3(2):163–175. doi:10.1038/nprot.2007.521. nprot.2007.521 [pii]

    Article  CAS  PubMed  Google Scholar 

  3. CLSI (2015) Performance standards for antimicrobial susceptibility testing; twenty-fifth informational supplement (M100-S25). Clinical and Laboratory Standards Institute, Wayne, PA

    Google Scholar 

  4. Munoz-Davila MJ, Roig M, Yague G, Blazquez A, Salvador C, Segovia M (2013) Comparative evaluation of Vitek 2 identification and susceptibility testing of urinary tract pathogens directly and isolated from chromogenic media. Eur J Clin Microbiol Infect Dis 32(6):773–780. doi:10.1007/s10096-012-1806-4

    Article  CAS  PubMed  Google Scholar 

  5. Cuny C, Witte W (2005) PCR for the identification of methicillin-resistant Staphylococcus aureus (MRSA) strains using a single primer pair specific for SCCmec elements and the neighbouring chromosome-borne orfX. Clin Microbiol Infect 11(10):834–837 . doi:10.1111/j.1469-0691.2005.01236.xCLM1236 [pii]

    Article  CAS  PubMed  Google Scholar 

  6. Brolund A, Wisell KT, Edquist PJ, Elfstrom L, Walder M, Giske CG (2010) Development of a real-time SYBRGreen PCR assay for rapid detection of acquired AmpC in enterobacteriaceae. J Microbiol Methods 82(3):229–233. doi:10.1016/j.mimet.2010.06.006. S0167-7012(10)00203-4 [pii]

    Article  CAS  PubMed  Google Scholar 

  7. Oviano M, Fernandez B, Fernandez A, Barba MJ, Mourino C, Bou G (2014) Rapid detection of enterobacteriaceae producing extended spectrum beta-lactamases directly from positive blood cultures by matrix-assisted laser desorption ionization-time of flight mass spectrometry. Clin Microbiol Infect 20(11):1146–1157. doi:10.1111/1469-0691.12729

    Article  CAS  PubMed  Google Scholar 

  8. Hrabak J, Chudackova E, Walkova R (2013) Matrix-assisted laser desorption ionization-time of flight (maldi-tof) mass spectrometry for detection of antibiotic resistance mechanisms: from research to routine diagnosis. Clin Microbiol Rev 26(1):103–114. doi:10.1128/CMR.00058-12. 26/1/103 [pii]

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Kempf M, Bakour S, Flaudrops C, Berrazeg M, Brunel JM, Drissi M, Mesli E, Touati A, Rolain JM (2012) Rapid detection of carbapenem resistance in Acinetobacter Baumannii using matrix-assisted laser desorption ionization-time of flight mass spectrometry. PLoS One 7(2):e31676. doi:10.1371/journal.pone.0031676. PONE-D-11-22833 [pii]

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Vilhelmsson SE, Tomasz A, Kristinsson KG (2000) Molecular evolution in a multidrug-resistant lineage of Streptococcus pneumoniae: emergence of strains belonging to the serotype 6B Icelandic clone that lost antibiotic resistance traits. J Clin Microbiol 38(4):1375–1381

    CAS  PubMed  PubMed Central  Google Scholar 

  11. Mezger A, Gullberg E, Goransson J, Zorzet A, Herthnek D, Tano E, Nilsson M, Andersson DI (2015) A general method for rapid determination of antibiotic susceptibility and species in bacterial infections. J Clin Microbiol 53(2):425–432. doi:10.1128/JCM.02434-14. JCM.02434-14 [pii]

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Nilsson M, Malmgren H, Samiotaki M, Kwiatkowski M, Chowdhary BP, Landegren U (1994) Padlock probes: circularizing oligonucleotides for localized DNA detection. Science 265(5181):2085–2088

    Article  CAS  PubMed  Google Scholar 

  13. Baner J, Nilsson M, Mendel-Hartvig M, Landegren U (1998) Signal amplification of padlock probes by rolling circle replication. Nucleic Acids Res 26(22):5073–5078. doi:gkb813 [pii]

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Lizardi PM, Huang X, Zhu Z, Bray-Ward P, Thomas DC, Ward DC (1998) Mutation detection and single-molecule counting using isothermal rolling-circle amplification. Nat Genet 19(3):225–232. doi:10.1038/898

    Article  CAS  PubMed  Google Scholar 

  15. Dahl F, Baner J, Gullberg M, Mendel-Hartvig M, Landegren U, Nilsson M (2004) Circle-to-circle amplification for precise and sensitive DNA analysis. Proc Natl Acad Sci U S A 101(13):4548–4553. doi:10.1073/pnas.0400834101. 0400834101 [pii]

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  16. Jarvius J, Melin J, Goransson J, Stenberg J, Fredriksson S, Gonzalez-Rey C, Bertilsson S, Nilsson M (2006) Digital quantification using amplified single-molecule detection. Nat Methods 3(9):725–727. doi:10.1038/Nmeth916

    Article  CAS  PubMed  Google Scholar 

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Correspondence to Dan I. Andersson .

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Mezger, A., Nilsson, M., Andersson, D.I. (2017). Rapid Antibiotic Susceptibility Testing for Urinary Tract Infections. In: Bishop-Lilly, K. (eds) Diagnostic Bacteriology. Methods in Molecular Biology, vol 1616. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7037-7_9

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  • DOI: https://doi.org/10.1007/978-1-4939-7037-7_9

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7035-3

  • Online ISBN: 978-1-4939-7037-7

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