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Ion-Mobility Mass Spectrometry for Lipidomics Applications

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Lipidomics

Part of the book series: Neuromethods ((NM,volume 125))

Abstract

Among lipidomics’ major challenges, there is the molecular complexity of the lipidome. State-of-the-art technology, such as ion mobility (IM) spectrometry, is a promising new tool for supporting lipidomics research. IM is a gas-phase electrophoretic technique that enables the separation of ions in the gas phase according to their charge, shape, and size. IM separation, which occurs on a time scale of milliseconds, is compatible with modern mass spectrometers with microsecond scan speeds. Thus, IM-mass spectrometry (MS) can be integrated into conventional lipidomics MS workflows to improve separation and enhance peak capacity, spectral clarity, and fragmentation specificity. Furthermore, IM allows for the determination of the collision cross section (CCS), an orthogonal physicochemical measure that can be used, together with accurate mass and fragmentation information, to increase the confidence of lipid identification. In recent years, the expanding sophistication of hardware and software products has enabled IM-MS to perform an increasingly important role in traditional lipidomic approaches. In this chapter, we present IM-MS procedures for lipidomics research.

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Acknowledgments

We would like to thank Drs. David Grant, Andrea Armirotti, Will Thompson, Michal Kliman, Hans Vissers, Kevin Giles, Jonathan Williams, Nick Tomczyk, and Suraj Dhungana for discussions we found most enlightening.

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Authors and Affiliations

  1. European Academy of Bolzano/Bozen, Center for Biomedicine, Via Galvani 31, 39100, Bolzano, Italy

    Giuseppe Paglia

  2. Waters Corporation, Milford, MA, USA

    Bindesh Shrestha & Giuseppe Astarita

  3. Department of Biochemistry and Molecular & Cellular Biology, Georgetown University, Washington, DC, USA

    Giuseppe Astarita

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  1. Giuseppe Paglia
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  2. Bindesh Shrestha
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  3. Giuseppe Astarita
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Correspondence to Giuseppe Astarita .

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Editors and Affiliations

  1. Metabolomics Unit, Lincoln Memorial University, Harrogate, Tennessee, USA

    Paul Wood

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Paglia, G., Shrestha, B., Astarita, G. (2017). Ion-Mobility Mass Spectrometry for Lipidomics Applications. In: Wood, P. (eds) Lipidomics. Neuromethods, vol 125. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6946-3_5

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  • DOI: https://doi.org/10.1007/978-1-4939-6946-3_5

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