Abstract
Generation of selective anti-G-protein coupled receptor (GPCR) antibodies is a challenging pursuit and the anti-histamine receptor antibodies are no exception. Furthermore, producing isoform-selective antibodies is even more of a challenge. The first anti-histamine H3 receptor (H3) antibodies were developed in 2001 and validated in 2007. The first two antibodies were raised against human H3 receptor sequences common to most human and rodent isoforms. This chapter describes how a panel of anti-hH3 receptor isoform-specific antibodies have been generated, characterized, and validated for selectivity [individual rH3R and hH3R isoform transient expression in human embryonic kidney (HEK) 293 cells, isoform peptide blockade, and the use of H3R knock-out (KO) mice], and utility for a range of immunobiochemical techniques (immunoblotting, immunohistochemical, and immunofluorescence techniques).
*These authors contributed equally in this chapter.
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Shenton, F.C., Lethbridge, N., Chazot, P.L. (2017). Development, Characterization, and Validation of Anti-Human H3 Receptor Isoform-Specific Antibodies. In: Tiligada, E., Ennis, M. (eds) Histamine Receptors as Drug Targets. Methods in Pharmacology and Toxicology. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6843-5_2
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DOI: https://doi.org/10.1007/978-1-4939-6843-5_2
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