Histamine Receptors as Drug Targets

1. Front Matter

   Pages i-xvi

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2. Methodologies to Identify and Analyze Genetic Polymorphisms for Human Histamine Receptors

   • Stephany Micallef, Astrid Sasse

   Pages 1-18

3. Development, Characterization, and Validation of Anti-Human H3 Receptor Isoform-Specific Antibodies

   • Fiona C. Shenton, Natasha Lethbridge, Paul L. Chazot

   Pages 19-47

4. Approaches to Recombinant Histamine H3/H4 Receptor Expression in Mammalian Cells

   • Tadeusz Karcz, Urszula Cichoń, Katarzyna Kieć-Kononowicz

   Pages 49-66

5. Histamine H2 Receptor Biased Signaling Methods

   • Natalia C. Fernández, Carina Shayo, Carlos Davio, Federico Monczor

   Pages 67-114

6. Ligand-Binding Kinetics on Histamine Receptors

   • Reggie Bosma, Tamara A. M. Mocking, Rob Leurs, Henry F. Vischer

   Pages 115-155

7. Methods to Study the Molecular Pharmacology of the Histamine H4 Receptor

   • Eléonore W. E. Verweij, Rob Leurs, Henry F. Vischer

   Pages 157-181

8. Chemiluminescence: A Sensitive Method for Detecting the Effects of Histamine Receptor Agonists/Antagonists on Neutrophil Oxidative Burst

   • Ondřej Vašíček, Tomáš Perečko, Viera Jančinová, Silvia Pažoureková, Radomír Nosáľ, Milan Číž

   Pages 183-208

9. Techniques Used in Pharmacological Evaluation of Histamine H4 Receptor Function on Native Human Eosinophils

   • Marek Grosicki, Katarzyna Kieć-Kononowicz

   Pages 209-232

10. Assessment of the Efficacy of Histamine Receptor Antagonists in Murine Models of Atopic Dermatitis

    • Kristine Roßbach, Hanna Köchling, Tomoki Fukuyama, Jenny Wilzopolski, Manfred Kietzmann, Wolfgang Bäumer

    Pages 233-262

11. Methodological Approaches to Study Histamine Pharmacology in the Kidney: From Cell Culture and Cell Imaging to Functional Assays and Electron Microscopy

    • Alessandro Pini, Eleonora Veglia, Cristina Grange, Arianna Carolina Rosa

    Pages 263-308

12. Histamine and the Kidney: In Vivo Animal Models

    • Alessandro Pini, Eleonora Veglia, Arianna Carolina Rosa

    Pages 309-352

13. Methodological Approaches to Investigate the Effects of Histamine Receptor Targeting Compounds in Preclinical Models of Breast Cancer

    • Diego J. Martinel Lamas, Melisa B. Nicoud, Helena Sterle, Graciela P. Cricco, Gabriela A. Martin, Graciela A. Cremaschi et al.

    Pages 353-387

14. Evaluation of Histidine Decarboxylase (HDC) Activity and Human Histamine Receptor 3 (H3R) and HDC mRNA Levels

    • Carlos Acosta-Andrade, José L. Urdiales, Ignacio Fajardo, Francisca Sánchez-Jiménez

    Pages 389-410

15. Cerebrospinal Fluid Markers in Neuroinflammation: The Paradigm of Optic Neuritis

    • Dimitrios P. Besinis, Maria C. Anagnostouli

    Pages 411-431

16. In Vivo Diagnostic Procedures for IgE-Mediated Allergic Disorders

    • Christopher Koulias, Xenophon Aggelides, Caterina Chliva, Michael Makris

    Pages 433-472

17. Dermatological Indications of Antihistamines

    • Chrysovalantis Korfitis, Efstathios Rallis, Dimitrios Rigopoulos

    Pages 473-487

18. Histamine Quantification in Human Blood Samples

    • Ekaterini Tiligada, Maria Kakolyri, Madeleine Ennis

    Pages 489-508

19. Back Matter

    Pages 509-517

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This book illustrates the current state-of-the-art in histamine research, with a focus on the appropriate methodologies to investigate the pharmacological properties and the therapeutic exploitation of HRs and their ligands. In addition, the range of techniques described provides an introduction to complementary cross-methodological disciplines beyond these fields. This multi-disciplinary approach is required to define the ‘decision gates’ that determine the development of more effective and safer therapeutic options for many forms of highly prevalent and debilitating diseases, such as asthma, dementias, dermatitis, and arthritis. Written for the Methods in Pharmacology and Toxicology series, chapters concentrate on practical, hands-on protocols from experts in the techniques.

Authoritative and thorough, Histamine Receptors as Drug Targets seeks to aid pharmacologists, biochemists, drug discovery researchers, molecular biologists, chemists, toxicologists, lab scientists, medical doctors, principle investigators, research scientists, lab directors and technicians, as well as graduate students around the world in pursuing the study of this vital scientific area.

• Histamine receptor (HR)
• Translational research
• Immune system
• Drug discovery
• Pharmacological properties
• Therapeutic applications
• Antihistamines

• Department of Pharmacology Medical School, National and Kapodistrian University of Athens, Athens, Greece

  Ekaterini Tiligada

• Centre for Experimental Medicine, School of Medicine, Dentistry and Biomedical Sciences, Queen’s University Belfast, Belfast, Northern Ireland, United Kingdom

  Madeleine Ennis

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