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Evaluation of Histidine Decarboxylase (HDC) Activity and Human Histamine Receptor 3 (H3R) and HDC mRNA Levels

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Histamine Receptors as Drug Targets

Part of the book series: Methods in Pharmacology and Toxicology ((MIPT))

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Abstract

Histamine plays pleiotropic roles in human pathophysiology that are dependent on both its ability to be synthetized in a given environment and the cell-specific combination of histamine receptors expressed in the different human cell types. At present, characterization of the molecular basis of the tissue-specific roles of the histamine metabolism-related elements requires further research efforts to fully explain the multiple roles that histamine plays in human pathophysiology and to develop new intervention strategies derived from this knowledge. In this chapter, we describe some methods used in our laboratory for many years to investigate histidine decarboxylase (HDC) and histamine receptors (HRs) in different human cell types and tissues. These are a radiolabeled method for HDC activity measurement and quantitative real-time polymerase chain reaction (qPCR) methods for quantification and transcript analyses of human HDC and histamine receptor type 3 (H3R).

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Acknowledgments

This work was supported by Grants SAF2011-26518 (MINECO, Spain), PAIDI Grant P10-CVI6585 (Andalusian Government). We also thank the support from CIBERER, as well as from the University of Málaga and Andalucia-Tech facilities. CIBERER is an initiative of Instituto de Salud Carlos III.

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Authors and Affiliations

  1. Departamento de Biología Molecular y Bioquímica, Andalucía Tech, Facultad de Ciencias, Universidad de Málaga, Campus Universitario de Teatinos, Malaga, 29071, Spain

    Carlos Acosta-Andrade, José L. Urdiales, Ignacio Fajardo & Francisca Sánchez-Jiménez

  2. Instituto de Biomedicina de Málaga, Malaga, Spain

    José L. Urdiales, Ignacio Fajardo & Francisca Sánchez-Jiménez

  3. CIBER de Enfermedades Raras, Instituto de Salud Carlos III, Malaga, Spain

    José L. Urdiales, Ignacio Fajardo & Francisca Sánchez-Jiménez

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  1. Carlos Acosta-Andrade
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  2. José L. Urdiales
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  3. Ignacio Fajardo
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  4. Francisca Sánchez-Jiménez
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Corresponding author

Correspondence to Francisca Sánchez-Jiménez .

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Editors and Affiliations

  1. Department of Pharmacology Medical School, National and Kapodistrian University of Athens, Athens, Greece

    Ekaterini Tiligada

  2. Centre for Experimental Medicine, School of Medicine, Dentistry and Biomedical Sciences, Queen’s University Belfast, Belfast, Northern Ireland, United Kingdom

    Madeleine Ennis

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Acosta-Andrade, C., Urdiales, J.L., Fajardo, I., Sánchez-Jiménez, F. (2017). Evaluation of Histidine Decarboxylase (HDC) Activity and Human Histamine Receptor 3 (H3R) and HDC mRNA Levels. In: Tiligada, E., Ennis, M. (eds) Histamine Receptors as Drug Targets. Methods in Pharmacology and Toxicology. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6843-5_13

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  • DOI: https://doi.org/10.1007/978-1-4939-6843-5_13

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