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In Vitro Assays to Identify Antibiotics Targeting DNA Metabolism

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Antibiotics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1520))

Abstract

DNA metabolism embodies a number of biochemical pathways, which include targets of clinically used antibiotics as well as those that are only being explored as potential targets for inhibitory compounds. We give an overview of representative cell-based and enzymatic assays suitable for high-throughput-driven search for novel DNA metabolism inhibitors of established and novel DNA metabolism targets in bacteria. The protocol for a colorimetric coupled primase-inorganic pyrophosphatase assay developed by our group is described in detail.

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Authors and Affiliations

  1. Department of Pharmaceutical Sciences, University of Kentucky, 789 S. Limestone Street, Lexington, KY, 40536, USA

    Allan H. Pang, Sylvie Garneau-Tsodikova & Oleg V. Tsodikov

Authors
  1. Allan H. Pang
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  2. Sylvie Garneau-Tsodikova
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  3. Oleg V. Tsodikov
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Corresponding author

Correspondence to Oleg V. Tsodikov .

Editor information

Editors and Affiliations

  1. Interfaculty Institute for Microbiology and Infection Medicine, Microbial Bioactive Compounds, University of Tübingen, Tübingen, Germany

    Peter Sass

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Pang, A.H., Garneau-Tsodikova, S., Tsodikov, O.V. (2017). In Vitro Assays to Identify Antibiotics Targeting DNA Metabolism. In: Sass, P. (eds) Antibiotics. Methods in Molecular Biology, vol 1520. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6634-9_11

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  • DOI: https://doi.org/10.1007/978-1-4939-6634-9_11

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6632-5

  • Online ISBN: 978-1-4939-6634-9

  • eBook Packages: Springer Protocols

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