Abstract
The identification of TIR1 as an auxin receptor combined with advanced biophysical instrumentation has led to the development of real-time activity assays for auxins. Traditionally, molecules have been assessed for auxinic activity using bioassays, and agrochemical compound discovery continues to be based on “spray and pray” technologies. Here, we describe the methodology behind an SPR-based assay that uses TIR1 and related F-box proteins with surface plasmon resonance spectrometry for rapid compound screening. In addition, methods for collecting kinetic binding data and data processing are given so that they may support programs for rational design of novel auxin ligands.
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Acknowledgement
The authors are grateful for support from BBSRC, UK, Award BB/L009366.
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Quareshy, M., Uzunova, V., Prusinska, J.M., Napier, R.M. (2017). Assaying Auxin Receptor Activity Using SPR Assays with F-Box Proteins and Aux/IAA Degrons. In: Kleine-Vehn, J., Sauer, M. (eds) Plant Hormones. Methods in Molecular Biology, vol 1497. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6469-7_15
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DOI: https://doi.org/10.1007/978-1-4939-6469-7_15
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Online ISBN: 978-1-4939-6469-7
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