Abstract
Identifying gene expression changes mediated by signaling pathways is necessary to determine mechanisms that cause phenotypic change. Recent advances in next-generation sequencing and informatic pipelines have streamlined the ability for laboratories to create and analyze transcriptomic data. Here, we describe the preparation of samples and transcriptomic analysis in order to determine gene expression programs regulated by RAS/ERK signaling.
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Acknowledgement
This work was supported by Research Scholar Award RSG-13-215-01-DMC from the American Cancer Society.
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Plotnik, J.P., Hollenhorst, P.C. (2017). Genome-Wide Analysis of RAS/ERK Signaling Targets. In: Jimenez, G. (eds) ERK Signaling. Methods in Molecular Biology, vol 1487. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6424-6_21
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DOI: https://doi.org/10.1007/978-1-4939-6424-6_21
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