ERK Signaling

Methods and Protocols

  • Gerardo Jimenez
Part of the Methods in Molecular Biology book series (MIMB, volume 1487)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Dariel Ashton-Beaucage, Marc Therrien
    Pages 1-21
  3. Zhihong Wang, Christine Candelora
    Pages 23-33
  4. Johannes F. Weijman, Stefan J. Riedl, Peter D. Mace
    Pages 53-63
  5. Tal Goshen-Lago, Dganit Melamed, Arie Admon, David Engelberg
    Pages 65-88
  6. Carme Caelles, Carles Bayod, Melisa Morcillo
    Pages 89-98
  7. Yuji Kubota, Mutsuhiro Takekawa
    Pages 99-111
  8. Rona Grossman, Ze’ev Paroush
    Pages 127-135
  9. Lorena Agudo-Ibañez, Piero Crespo, Berta Casar
    Pages 151-162
  10. Raffaele Strippoli, Asier Echarri, Miguel Angel del Pozo
    Pages 163-174
  11. Denise A. Berti, Rony Seger
    Pages 175-194
  12. Yannick Blum, Rafael D. Fritz, Hyunryul Ryu, Olivier Pertz
    Pages 203-221
  13. Hiroaki Hirata, Mukund Gupta, Sri Ram Krishna Vedula, Chwee Teck Lim, Benoit Ladoux, Masahiro Sokabe
    Pages 223-234
  14. Marc K. Saba-El-Leil, Christophe Frémin, Sylvain Meloche
    Pages 243-253
  15. Athina-Myrto Chioni, Rabia Tayba Bajwa, Richard Grose
    Pages 255-267

About this book

Introduction

This volume provides a collection of techniques and approaches for the study of ERK signaling. It begins with a historical perspective of genetic and molecular discoveries, followed by chapters covering specific topics in a broad range of experimental systems, including in vitro assays of EGFR and ERK activities; proteomic and genome-wide analyses of ERK signaling targets; cell biological, genetic, quantitative and imaging approaches in cells and model organisms; and integrated mathematical modeling of the ERK pathway. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, ERK Signaling: Methods and Protocols will aid and stimulate further advances in the vibrant field of ERK signaling.

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Keywords

signaling pathways ERK2 protein complexes mutants of Mpk1/ERK SUMO-Modified MEK direct MAPK/ERK substrates compartmentalization by subcellular fractionation ERK pathway/caveolin1 interactions nuclear translocation Drosophila CRISPR-Cas9

Editors and affiliations

  • Gerardo Jimenez
    • 1
  1. 1.Institut de Biologia Molecular de Barcelona-CSICParc Científic de BarcelonaBarcelonaSpain

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-6424-6
  • Copyright Information Springer Science+Business Media New York 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6422-2
  • Online ISBN 978-1-4939-6424-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book