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Protein Stability: Enhancement and Measurement

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Protein Chromatography

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1485))

Abstract

This article defines protein stability, emphasizes its importance and surveys the field of protein stabilization, with summary reference to a selection of 2009–2015 publications. One can enhance stability by, in particular, protein engineering strategies and by chemical modification (including conjugation) in solution. General protocols are set out on how to measure a given protein’s (1) kinetic thermal stability, and (2) oxidative stability, and (3) how to undertake chemical modification of a protein in solution.

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Authors and Affiliations

  1. School of Biotechnology, Dublin City University, Glasnevin, Dublin 9, Ireland

    Ciarán Ó’Fágáin

  2. National Centre for Sensor Research, Dublin City University, Glasnevin, Dublin 9, Ireland

    Ciarán Ó’Fágáin

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  1. Ciarán Ó’Fágáin
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Correspondence to Ciarán Ó’Fágáin .

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Editors and Affiliations

  1. School of Biotechnology, Dublin City University, Dublin, Ireland

    Dermot Walls

  2. Department of Applied Sciences, Dundalk Institute of Technology, Dublin, Ireland

    Sinéad T. Loughran

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Ó’Fágáin, C. (2017). Protein Stability: Enhancement and Measurement. In: Walls, D., Loughran, S. (eds) Protein Chromatography. Methods in Molecular Biology, vol 1485. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6412-3_7

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  • DOI: https://doi.org/10.1007/978-1-4939-6412-3_7

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6410-9

  • Online ISBN: 978-1-4939-6412-3

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