Protein Chromatography

Methods and Protocols

  • Dermot Walls
  • Sinéad T. Loughran

Part of the Methods in Molecular Biology book series (MIMB, volume 1485)

Table of contents

  1. Front Matter
    Pages i-x
  2. Overview Chapters

    1. Front Matter
      Pages 1-1
    2. Dermot Walls, Gary Cooney, Sinéad T. Loughran
      Pages 3-14
    3. Ciarán Ó’Fágáin, Philip M. Cummins, Brendan F. O’Connor
      Pages 15-25
    4. Jenny Fitzgerald, Paul Leonard, Elaine Darcy, Shikha Sharma, Richard O’Kennedy
      Pages 27-51
    5. Barry J. Ryan, Gary T. Henehan
      Pages 53-69
    6. William J. J. Finlay, Laird Bloom, Joanne Grant, Edward Franklin, Deirdre Ní Shúilleabháin, Orla Cunningham
      Pages 85-99
    7. Ciarán Ó’Fágáin
      Pages 101-129
    8. Sinéad T. Loughran, Dermot Walls
      Pages 131-156
  3. Protocols Chapters

    1. Front Matter
      Pages 157-157
    2. Ciarán Ó’Fágáin, Keith Colliton
      Pages 159-190
    3. Barry J. Ryan, Gemma K. Kinsella
      Pages 191-208
    4. Philip M. Cummins, Keith D. Rochfort, Brendan F. O’Connor
      Pages 209-223
    5. Eva M. Campion, Sinéad T. Loughran, Dermot Walls
      Pages 225-255
    6. Mario Lebendiker, Tsafi Danieli
      Pages 257-273
    7. Sinéad T. Loughran, Ronan T. Bree, Dermot Walls
      Pages 275-303
    8. Elaine Darcy, Paul Leonard, Jenny Fitzgerald, Martin Danaher, Hui Ma, Richard O’Kennedy
      Pages 305-318
    9. William J. J. Finlay, Laird Bloom, Sreeja Varghese, Bénédicte Autin, Orla Cunningham
      Pages 319-338
    10. Paul Leonard, Stephen Hearty, Hui Ma, Richard O’Kennedy
      Pages 339-354

About this book

Introduction

This second edition expands on the previous edition with new chapters that are suitable for newcomers, as well as more detailed chapters that cover protein stability and storage, avoiding proteolysis during chromatography, protein quantitation methods including immuno-qPCR, and the challenges that scale-up of production poses to the investigator. Many of the chapters also discuss generation and purification of recombinant proteins, recombinant antibody production, and the tagging of proteins as a means to enhance their solubility and simplify their purification on an individual scale or in high-throughput systems. This book also provides readers with chapters that describe not just the more commonly used methods, but also recently developed approaches such as proteomic/mass spectrometric techniques and Lectin-based affinity chromatography. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Protein Chromatography: Methods and Protocols, Second Edition is a valuable resource for anyone who is interested in the field of protein chromatography.

Keywords

protein stability and storage avoiding proteolysis during chromatography protein quantitation immuno-qPCR purification of recombinant proteins recombinant antibody production high-throughput systems physicochemical properties of the target protein ion-exchange immunoaffinity chromatography

Editors and affiliations

  • Dermot Walls
    • 1
  • Sinéad T. Loughran
    • 2
  1. 1.School of BiotechnologyDublin City UniversityDublinIreland
  2. 2.Department of Applied SciencesDundalk Institute of TechnologyDublinIreland

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-6412-3
  • Copyright Information Springer Science+Business Media New York 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6410-9
  • Online ISBN 978-1-4939-6412-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book