Abstract
Models of skeletal muscle injury in animal models are invaluable tools to assess muscle stem cell (MuSC)-mediated tissue repair. The optimization and comprehensive evaluation of these approaches have greatly improved our ability to assess MuSC regenerative potential. Here we describe the procedures for skeletal muscle injury with notexin and BaCl2 and assessment of the dynamics of tissue regeneration.
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Acknowledgements
This work was supported by the US National Institutes of Health (NIH) grants R01 AR064873, R03 AR063328 and P30 AR06130303 to AS, and US National Institutes of Health (NIH) grant F31 AR065923-01 to MT. We thank Kenny Venegas and Buddy Charbono for technical support.
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Tierney, M.T., Sacco, A. (2016). Inducing and Evaluating Skeletal Muscle Injury by Notexin and Barium Chloride. In: Kyba, M. (eds) Skeletal Muscle Regeneration in the Mouse. Methods in Molecular Biology, vol 1460. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-3810-0_5
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DOI: https://doi.org/10.1007/978-1-4939-3810-0_5
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